Detailed information of TA system TA144378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2670477..2671027
• Replicon: chromosome
• Accession: NZ_CP046527
• Organism: Escherichia coli strain RM19259

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: GN312_RS13430
• Protein ID: WP_001160975.1
• Coordinates: 2670713..2671027 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: GN312_RS13425
• Protein ID: WP_000125566.1
• Coordinates: 2670477..2670710 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN312_RS13400	2665551..2665838	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
GN312_RS13405	2665896..2667227	+	1332	WP_001183177.1	MFS transporter	-
GN312_RS13410	2667335..2667865	+	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
GN312_RS13415	2667858..2669720	+	1863	WP_000377184.1	glutathione-regulated potassium-efflux system protein KefC	-
GN312_RS13420	2669912..2670391	+	480	WP_000624372.1	type 3 dihydrofolate reductase	-
GN312_RS13425	2670477..2670710	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GN312_RS13430	2670713..2671027	+	315	WP_001160975.1	CcdB family protein	Toxin
GN312_RS13435	2671024..2671872	-	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
GN312_RS13440	2671879..2672256	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
GN312_RS13445	2672259..2673080	-	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
GN312_RS13450	2673077..2674066	-	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
GN312_RS13455	2674066..2675352	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T144378 WP_001160975.1 NZ_CP046527:2670713-2671027 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T144378 NZ_CP061932:92572-92859 [Klebsiella michiganensis]
ATGACTTATACGGTCAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAGCTGGATAAGTCCATTCAGCAGCAGTTTGC
GAAGAAACTGAAGAAATGCTGTGACGAACCACATATCCCCTCTGCAAAGCTGAGAGGAATAAAGGACTGTTATAAAATTA
AACTTCGTGCATCAGGTTTTCGCCTGGTGTATCAGGTGATAGATGATCAATTAATTATCGCGGTTGTTGCTGTTGGAAAG
CGTGAACGCAGTGACGTGTACAATCTGGCCAGCGAAAGGATGAGATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT144378 WP_000125566.1 NZ_CP046527:2670477-2670710 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT144378 NZ_CP061932:92324-92575 [Klebsiella michiganensis]
ATGCCCAACATTATTCTGAGTGACACAAGCGCGAGCATCAGCGAACTTAAAAAAAACCCGATGGCGACGGTCAGTGCCGG
CGACGGCTTCCCGGTTGCTATTCTGAACCGTAACCAGCCCGCTTTTTACTGTATCCCGGCAGAGCTTTACGAGAAGATGC
TCGATGCCCTAGATGACCAGGAACTGGCAAAGCTGGTTTTAGAACGCAGTAACCAGCAGCTGCTTGACGTGGACCTGGAC
ACTTATCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9