Detailed information of TA system TA144373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 2422713..2423308
• Replicon: chromosome
• Accession: NZ_CP046527
• Organism: Escherichia coli strain RM19259

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: GN312_RS12390
• Protein ID: WP_000239579.1
• Coordinates: 2422958..2423308 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: GN312_RS12385
• Protein ID: WP_001223210.1
• Coordinates: 2422713..2422964 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN312_RS12375	2418378..2422157	+	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-
GN312_RS12380	2422160..2422501	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
GN312_RS12385	2422713..2422964	+	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
GN312_RS12390	2422958..2423308	+	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
GN312_RS12395	2423389..2423919	-	531	WP_000055075.1	inorganic diphosphatase	-
GN312_RS12400	2424229..2425185	+	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
GN312_RS28005	2425325..2426827	+	1503	Protein_2302	sugar ABC transporter ATP-binding protein	-
GN312_RS12410	2426841..2427863	+	1023	WP_001301928.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T144373 WP_000239579.1 NZ_CP046527:2422958-2423308 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T144373 NZ_CP061930:3477754-3478011 [Klebsiella michiganensis]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCACATCCGCGATGCATC
AGGCCGCGTCACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAGCTGATTAAGTCCGTCAACGGACAGCCCTACCGCATCAATACCACCGGGCTGAATAACGTCCGCGCA
CAGCTGGACAATCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT144373 WP_001223210.1 NZ_CP046527:2422713-2422964 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT144373 NZ_CP061930:3478022-3478573 [Klebsiella michiganensis]
ATGACAAACCACAGCTTCACTTTTCATGTCGCCTCTGAAGGCGATACCGATGACATTCTCGACGTCGAAACCCGGGCTTT
CGGCTACAGCAAAGAGGCCAGGCTGGTGGCCGATTTGCTCAACGATGAGAGCGCCTGCCCGACGCTATCGCTGCTGGCCA
GGCATAACGGAGAGGCGGTTGGGCATATTTTATTCACCCGGGCGACGTTCAAAGGGGAGCCGGACTCGCCGATGATGCAC
ATCCTTGCTCCCCTGGCGGTGGTGCCGGAATACCAGGGCGTGGGCGTTGGCGGAGAGCTTATCCGGCACGGCATTGAGCA
GTTGAAAGCAATGGGAAGCCAGATGGTATTTGTTCTGGGCCACGCCGCTTACTACCCCAGGCACGGGTTTGAGCCATGCG
CGGGAGATAAAGGCTATCCGGCGCCTTATCCGATTCCGGAGGCGCATAAAGCCTGCTGGATGCTGCAGCGGCTTTCGTCT
CAGCCTCTGGGACGAACGGGCCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8