Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2325837..2326334 Replicon chromosome
Accession NZ_CP046527
Organism Escherichia coli strain RM19259

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GN312_RS11890 Protein ID WP_001173343.1
Coordinates 2326161..2326334 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GN312_RS11885 Protein ID WP_000398619.1
Coordinates 2325837..2326133 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GN312_RS11865 2322568..2323287 - 720 WP_000611288.1 two-component system response regulator DcuR -
GN312_RS11870 2323284..2324915 - 1632 WP_001216477.1 sensor histidine kinase -
GN312_RS11875 2325096..2325326 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
GN312_RS11880 2325338..2325610 + 273 WP_000405647.1 N-acetyltransferase -
GN312_RS11885 2325837..2326133 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GN312_RS11890 2326161..2326334 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GN312_RS11895 2326453..2327970 - 1518 WP_001295074.1 lysine--tRNA ligase -
GN312_RS11900 2328207..2329664 - 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T144372 WP_001173343.1 NZ_CP046527:2326161-2326334 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T144372 NZ_CP061930:3020273-3020560 [Klebsiella michiganensis]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAAGAAGTAATCTAAGAAAACATGGAATCCGGTTTGAGGAAGCGGT
GCTCGTCTTTGACGATCCCCGGCATCTTTCCCGACAGGATCGTTATGAGAATGGTGAATATCGCTGGCAAACTCTCGGCC
TCGTTCATGGGATCATTGTTATCATGGTTGCGCATTCGGTCCGTTTTGAAAGTGGAACAGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAGCAAAGAGAGAAATCGTTATGAACATGGTTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT144372 WP_000398619.1 NZ_CP046527:2325837-2326133 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT144372 NZ_CP061930:3020547-3020849 [Klebsiella michiganensis]
ATGAACATGGTTAAACATAAACGTGACAATCCGGATACGCTTACGGCTAAGCGAGACGATGAGCTTAAAGCGCTGGCCAA
CAAAACGGATGATGAAATCGATTATAGCGGTATTCCCGCCACAGACGACGCACAGTGGTCAGATGCCGTACGCGGTAAAT
TTTACCGACCGCTAAAAACTCAGGCTTCTGTCCGTATTGATGCCGATATTATGGAGTGGCTTAAACGTCCCGGGAAAGGT
TATCAAACGCGACTGAATGCGATCTTAAGAGAAGCGATGATTCGCGATCTTAACAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References