Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1506110..1506741 Replicon chromosome
Accession NZ_CP046527
Organism Escherichia coli strain RM19259

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag GN312_RS08125 Protein ID WP_001259385.1
Coordinates 1506466..1506741 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag GN312_RS08120 Protein ID WP_000593555.1
Coordinates 1506110..1506469 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GN312_RS08090 1501222..1501695 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
GN312_RS08095 1501692..1501973 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
GN312_RS08100 1502050..1503408 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
GN312_RS08105 1503401..1504909 + 1509 WP_001301773.1 carbohydrate kinase -
GN312_RS08110 1504899..1505168 + 270 WP_000933060.1 HPr family phosphocarrier protein -
GN312_RS08115 1505200..1506060 + 861 WP_001131788.1 class II aldolase -
GN312_RS08120 1506110..1506469 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GN312_RS08125 1506466..1506741 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
GN312_RS08130 1506814..1507938 - 1125 WP_001301659.1 ABC transporter permease -
GN312_RS08135 1507938..1510673 - 2736 WP_000149110.1 ribosome-associated ATPase/putative transporter RbbA -
GN312_RS08140 1510670..1511737 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T144369 WP_001259385.1 NZ_CP046527:c1506741-1506466 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T144369 NZ_CP061930:c1484887-1484402 [Klebsiella michiganensis]
ATGATCTCCACACCAGAGCCGCTTCATGCCGGGCACATTCTAACCCCGTTCTGCTGCGGCGTGGATTCTATGGATAACTG
GCTGAAACAGCGGGCGATGAAGAACCAGCTTTCTGGCGCGTCCCGTACGTTTGTCTGCTGCCACGATGCTAAGGTGATGG
CTTACTACTCGCTGGCGTCCAGCGCGGTAACGACAAACACCGCCCCCGGCCGCTTTCGTCGCAATATGCCCGATCCGATC
CCGGTTGTAGTGCTGGGACGTCTGGCGGTGGATCGGTCGCTACATGGACAAGGCGTTGGGCGGGCGCTGGTGAGGGATGC
CGGACTGCGGGTGATTCAGGTGGCGGAAACTATCGGTATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGCGGG
AGTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT144369 WP_000593555.1 NZ_CP046527:c1506469-1506110 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT144369 NZ_CP061930:c1485177-1484884 [Klebsiella michiganensis]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAATTTGCGCATCAAACCCGCTGAGCGCGATCTTATCGA
TCGGGCAGCGAAAGCCAGAGGGAAAAACCGCACAGATTTTGTGCTAGAAGCTGCCCGCGCTGCTGCCGAGGAAGCGTTGA
TCGAGCAGCGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTTGCCCGTCTGGATCAGGCTCCTGCGCCCAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References