Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 1463510..1464310 Replicon chromosome
Accession NZ_CP046527
Organism Escherichia coli strain RM19259

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag GN312_RS07875 Protein ID WP_000342448.1
Coordinates 1463510..1464037 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag GN312_RS07880 Protein ID WP_001277108.1
Coordinates 1464044..1464310 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GN312_RS07850 1458585..1459352 - 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
GN312_RS07855 1459349..1460626 - 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
GN312_RS07860 1460623..1461549 - 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
GN312_RS07865 1461597..1462706 - 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
GN312_RS07870 1463130..1463513 + 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
GN312_RS07875 1463510..1464037 - 528 WP_000342448.1 GNAT family N-acetyltransferase Toxin
GN312_RS07880 1464044..1464310 - 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
GN312_RS07885 1464460..1465563 - 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
GN312_RS07890 1465834..1466688 - 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
GN312_RS07895 1466933..1467991 - 1059 WP_001042003.1 permease-like cell division protein FtsX -
GN312_RS07900 1467984..1468652 - 669 WP_000617723.1 cell division ATP-binding protein FtsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T144368 WP_000342448.1 NZ_CP046527:c1464037-1463510 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T144368 NZ_CP061930:c1337439-1337029 [Klebsiella michiganensis]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCGTGGCGAGCGCAGTGGTTTTCGCTGCTGATGCACGGCGTGGTGGCGGC
GCTGGTGCTGCTGATGCCCTGGCCGCTGAGCTATACCCCGCTGTGGCTGATACTGCTGTCGCTGGTGGTGTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCAGGGAGAAATCAAGCTGCTGATCGACTCCCGTCTGCGCTGGCAAAAGGCG
GAGTGGGACATCATCGGCACCCCGTGGGTGATTAACAGCGGAATGCTGCTGCGTCTGCGGAATACGGAAAACCAACGAAC
CCAGCATTTGTGGGTCGCGGCGGATAGCATGGACGCGGGAGAATGGCGAGACCTGCGTCGGCTGGTGTTGCTAAAACCGA
CGCAAGAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT144368 WP_001277108.1 NZ_CP046527:c1464310-1464044 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT144368 NZ_CP061930:c1337686-1337420 [Klebsiella michiganensis]
ATGGACATTAACAATAAAGCCCGCATCCACTGGGCATGCCGTCGAGGTATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTCGAGCATGAGTACGACACGCTCAGCGATGGCGATAAAGCGTTATTCATCCGCCTGCTGGAAAACGACGATCCGGATC
TGTTCAACTGGTTGATGAATCATGGCGAGCCGGCTGATGCAGAACTGCAGCGGATGGTAAAATTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References