Detailed information of TA system TA144367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 1414682..1415436
• Replicon: chromosome
• Accession: NZ_CP046527
• Organism: Escherichia coli strain RM19259

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: GN312_RS07655
• Protein ID: WP_001301452.1
• Coordinates: 1414951..1415436 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: Q8X700
• Locus tag: GN312_RS07650
• Protein ID: WP_000801912.1
• Coordinates: 1414682..1414960 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN312_RS07645	1411910..1414615	+	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-
GN312_RS07650	1414682..1414960	+	279	WP_000801912.1	DUF1778 domain-containing protein	Antitoxin
GN312_RS07655	1414951..1415436	+	486	WP_001301452.1	GNAT family N-acetyltransferase	Toxin
GN312_RS07660	1415486..1416514	-	1029	WP_000827117.1	RNA 3'-terminal phosphate cyclase	-
GN312_RS07665	1416518..1417744	-	1227	WP_001105473.1	RNA-splicing ligase RtcB	-
GN312_RS07670	1417932..1419530	+	1599	WP_001232889.1	DNA-binding transcriptional regulator RtcR	-
GN312_RS07675	1419512..1420270	-	759	WP_001302007.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17650.57 Da
• Isoelectric Point: 9.4066

>T144367 WP_001301452.1 NZ_CP046527:1414951-1415436 [Escherichia coli]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T144367 NZ_CP061930:c104198-103866 [Klebsiella michiganensis]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTGGATCCGATTGCAGGCCACGAGCAAAGCGGAAAACGTCCGGTGCTTAT
CGTATCGAAGGCATCGTTTAACAAGCTGACCCGGTTACCCGTTGTTGTTCCCATCACCAGCGGTGGAAATTTTGCTCGCA
CGGCAGGTTTCACCGTTTCACTGGAAGGGGCAGGGACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGCTGCCCGGAACGGAAAGCGGCTGGAAAGCATACCAGACGCTGTGGTAAATGAAGTGCTTGCCAGGCTGGATGC
AATTTTAAGTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10420.94 Da
• Isoelectric Point: 4.6336

>AT144367 WP_000801912.1 NZ_CP046527:1414682-1414960 [Escherichia coli]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT144367 NZ_CP061930:c104456-104199 [Klebsiella michiganensis]
ATGTATACCACTCGCCTGAAAAAGGTCGGCGGATCCATCATGTTGGCGGTTCCTCCCGCCGTGCTCAAAACGCTGGAGCT
GTCGACGGACAGCGAAGTCGGCATGACCATTAATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCTCATTATTCTC
TTGAGGAGCTGCTGGCGCAGTGCGATCCGCACGCCGAAATGAGCGAAGAGGATAGGGAATGGATTGATGCTCCTGCGGTG
GGCAAGGAGATCCTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U9LHB9