Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 923971..924625 Replicon chromosome
Accession NZ_CP046527
Organism Escherichia coli strain RM19259

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag GN312_RS05150 Protein ID WP_000244781.1
Coordinates 923971..924378 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GN312_RS05155 Protein ID WP_000354046.1
Coordinates 924359..924625 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GN312_RS05130 919928..921661 - 1734 WP_000813185.1 single-stranded-DNA-specific exonuclease RecJ -
GN312_RS05135 921667..922377 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GN312_RS05140 922402..923298 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GN312_RS05145 923410..923931 + 522 WP_001055874.1 flavodoxin FldB -
GN312_RS05150 923971..924378 - 408 WP_000244781.1 protein YgfX Toxin
GN312_RS05155 924359..924625 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GN312_RS05160 924868..925848 + 981 WP_000886053.1 tRNA-modifying protein YgfZ -
GN312_RS05165 925925..926584 - 660 WP_000250274.1 hemolysin III family protein -
GN312_RS05170 926748..927059 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
GN312_RS05175 927104..928537 + 1434 WP_001310226.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T144364 WP_000244781.1 NZ_CP046527:c924378-923971 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T144364 NZ_CP061915:2036-2317 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACATGAT
GGCCGCCATTGAACTGGACGAACGAATTGGTTATTCAGCCAGCAGCCTGGCCTGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGAGTATTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT144364 WP_000354046.1 NZ_CP046527:c924625-924359 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT144364 NZ_CP061915:1770-2039 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGGGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References