Detailed information of TA system TA144362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 59212..59771
• Replicon: plasmid pRM19259
• Accession: NZ_CP046526
• Organism: Escherichia coli strain RM19259

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: GN312_RS00335
• Protein ID: WP_000421248.1
• Coordinates: 59496..59771 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: GN312_RS00330
• Protein ID: WP_001178089.1
• Coordinates: 59212..59496 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN312_RS00290	54404..54964	+	561	WP_000840472.1	fertility inhibition protein FinO	-
GN312_RS00295	55097..55309	+	213	WP_001302189.1	ANR family transcriptional regulator	-
GN312_RS00300	55554..56015	+	462	WP_001233856.1	thermonuclease family protein	-
GN312_RS00305	56061..56270	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
GN312_RS00310	56308..56646	+	339	WP_000766796.1	hypothetical protein	-
GN312_RS00315	56886..57140	+	255	WP_000083831.1	replication regulatory protein RepA	-
GN312_RS00320	57376..57450	+	75	WP_001370046.1	RepA leader peptide Tap	-
GN312_RS00325	57443..58300	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
GN312_RS00330	59212..59496	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
GN312_RS00335	59496..59771	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GN312_RS00340	59866..60072	+	207	WP_001105064.1	hypothetical protein	-
GN312_RS00345	60172..60387	-	216	Protein_65	transposase	-
GN312_RS00350	60431..60997	-	567	Protein_66	IS3-like element IS600 family transposase	-
GN312_RS00355	60997..61413	-	417	Protein_67	IS91 family transposase	-
GN312_RS00360	61413..61598	-	186	WP_001302179.1	hypothetical protein	-
GN312_RS00365	61775..63985	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
GN312_RS00370	64029..64418	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hlyC / hlyA / hlyB / hlyD / toxB / espP / stcE / exeE / exeG / hlyC	1..98304	98304

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T144362 WP_000421248.1 NZ_CP046526:59496-59771 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T144362 NZ_CP061914:c4472328-4472155 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT144362 WP_001178089.1 NZ_CP046526:59212-59496 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT144362 NZ_CP061914:c4472651-4472355 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8