Detailed information of TA system TA144355

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii-AbiEi
• Location: 824449..825881
• Replicon: chromosome
• Accession: NZ_CP046521
• Organism: Streptococcus canis strain HL_100

Toxin (Protein)

• Gene name: abiEi
• Uniprot ID: Q8DZ35
• Locus tag: GE024_RS04190
• Protein ID: WP_001030302.1
• Coordinates: 824449..825294 (-)
• Length: 282 a.a.

Antitoxin (Protein)

• Gene name: abiEii
• Uniprot ID: Q8DZ36
• Locus tag: GE024_RS04195
• Protein ID: WP_000037239.1
• Coordinates: 825291..825881 (-)
• Length: 197 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GE024_RS04180	820541..821560	+	1020	WP_093998679.1	type IV secretion system protein VirB4	-
GE024_RS04185	821562..824363	+	2802	WP_156667685.1	CHAP domain-containing protein	-
GE024_RS04190	824449..825294	-	846	WP_001030302.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GE024_RS04195	825291..825881	-	591	WP_000037239.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(M)	-	761720..921260	159540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (33-257)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (6-50)

Sequences

Toxin

Protein

• Length: 282 a.a.
• Molecular weight: 32414.90 Da
• Isoelectric Point: 5.2111

>T144355 WP_001030302.1 NZ_CP046521:c825294-824449 [Streptococcus canis]
MNKAKLTALCHKISKNTGLTFNSVMTYYFLEVILKKLSQSSYSNHYIFKGGFLLSNVIGVESRSTVDIDFLFHQITLSEE
TVKQQLKEILADSEEGISFVIQSITTIKESDDYGGYRATISCQLENIKQVIHLDIATGDVVTPQPITYDYKAIFDEDNFP
IIAYTIETILAEKLQTIYSRNFLNSRSKDFYDVYILSKLKKKDIDFNQLKNACQRTFSYRETELDFEKIIELLERFKSDP
TQNQQWQNYSKKYSYTKGISLANVLDEMISLITIIISTDSD

Nucleotide

• Length: 846 bp

>T144355 NZ_CP061914:c2615165-2614989 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 197 a.a.
• Molecular weight: 23074.63 Da
• Isoelectric Point: 7.8056

>AT144355 WP_000037239.1 NZ_CP046521:c825881-825291 [Streptococcus canis]
MSKKEILLDFIEKNNGIVTNKDCKALGIPTIYLTRLEKEGIIFRVEKGIFLTQNGDYDEYYFFQYRFPKAIFSYISALYL
QQFTDEIPQYFDVTVPRGYRFNTPPANLNIHFVSKEYSELGMTTVPTPMGNNVRVYDFERIICDFVIHREKIDSELFVKT
LQSYGNYPKKNLAKLYEYATKMNTLEKVKQTLEVLI

Nucleotide

• Length: 591 bp

>AT144355 NZ_CP061914:c2614943-2614527 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A8D822

Antitoxin

• AlphaFold DB: A0A2I7ZET8