Detailed information of TA system TA144353

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/PIN-RelB
• Location: 1344066..1344946
• Replicon: chromosome
• Accession: NZ_CP046514
• Organism: Bifidobacterium longum strain JSRL02

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F8APN2
• Locus tag: GN236_RS05925
• Protein ID: WP_007052625.1
• Coordinates: 1344066..1344557 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F8APN1
• Locus tag: GN236_RS05930
• Protein ID: WP_007057212.1
• Coordinates: 1344554..1344946 (-)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN236_RS05905	1339921..1340820	-	900	WP_007052631.1	Bax inhibitor-1/YccA family protein	-
GN236_RS05910	1340989..1341129	+	141	Protein_1117	response regulator transcription factor	-
GN236_RS05915	1341170..1341811	+	642	WP_029679476.1	GNAT family N-acetyltransferase	-
GN236_RS05920	1341901..1344006	+	2106	WP_156625172.1	DUF262 domain-containing protein	-
GN236_RS05925	1344066..1344557	-	492	WP_007052625.1	type II toxin-antitoxin system VapC family toxin	Toxin
GN236_RS05930	1344554..1344946	-	393	WP_007057212.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
GN236_RS05935	1345091..1347790	-	2700	WP_058062651.1	aconitate hydratase AcnA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1253002..1410889	157887

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-146)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (6-61)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 18531.70 Da
• Isoelectric Point: 6.2348

>T144353 WP_007052625.1 NZ_CP046514:c1344557-1344066 [Bifidobacterium longum]
MRQSKQVSILCDTNILIYYMLCTPRFIKDVMKLFERCVEREVKLSCASLSLKDVFYITPERFKERFMNDESMVVATTAKL
ILPEVAWSGISKLKELMTIVPIGAEECELAQSLRHAHNDFEDDLIIAAAQQSGATYVVTYDKKLAKHFPEYCKTPAEIVA
ALA

Nucleotide

• Length: 492 bp

>T144353 NZ_CP061914:2188267-2188370 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 15147.29 Da
• Isoelectric Point: 5.8624

>AT144353 WP_007057212.1 NZ_CP046514:c1344946-1344554 [Bifidobacterium longum]
MTMVQMNVRIDAKLKNEVEEVLKGKGVSVSDVIRSLWLYIADRKEVPALETTAEEQAREAEKQRKLKLIREGAGYVHKEL
VKAGLISEDTDLMEGLTYKQFRDEIADERLKEYRHQSDYARYLEKERSLS

Nucleotide

• Length: 393 bp

>AT144353 NZ_CP061914:c2188408-2188137 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: F8APN2

Antitoxin

• AlphaFold DB: F8APN1