Detailed information of TA system TA144350

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 2848111..2849863
• Replicon: chromosome
• Accession: NZ_CP046508
• Organism: Variovorax sp. RKNM96

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: GNX71_RS13125
• Protein ID: WP_206178718.1
• Coordinates: 2848111..2848983 (+)
• Length: 291 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: GNX71_RS13130
• Protein ID: WP_206178719.1
• Coordinates: 2849027..2849863 (+)
• Length: 279 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNX71_RS13100	2843181..2844221	+	1041	WP_206178713.1	2-dehydropantoate 2-reductase	-
GNX71_RS13105	2844276..2845535	+	1260	WP_206178714.1	D-amino acid dehydrogenase	-
GNX71_RS13110	2845714..2846700	+	987	WP_206178715.1	tripartite tricarboxylate transporter substrate binding protein	-
GNX71_RS13115	2846709..2847524	+	816	WP_206178716.1	SDR family oxidoreductase	-
GNX71_RS13120	2847555..2848103	+	549	WP_206178717.1	cupin domain-containing protein	-
GNX71_RS13125	2848111..2848983	+	873	WP_206178718.1	alpha/beta fold hydrolase	Toxin
GNX71_RS13130	2849027..2849863	+	837	WP_206178719.1	helix-turn-helix domain-containing protein	Antitoxin
GNX71_RS13135	2849869..2850708	+	840	WP_206179456.1	SDR family oxidoreductase	-
GNX71_RS13140	2850701..2852401	+	1701	WP_206178720.1	thiamine pyrophosphate-binding protein	-
GNX71_RS13145	2852422..2853219	+	798	WP_206178721.1	aspartate dehydrogenase	-
GNX71_RS13150	2853230..2854705	+	1476	WP_206178722.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (48-282)

Antitoxin

• Transcription regulator IclR, N-terminal (20-69)

Sequences

Toxin

Protein

• Length: 291 a.a.
• Molecular weight: 29930.42 Da
• Isoelectric Point: 6.4803

>T144350 WP_206178718.1 NZ_CP046508:2848111-2848983 [Variovorax sp. RKNM96]
MSDAMATDALMLPASELALLDDRFPARNVPVGGGAVVSVRERGTGPAIVCLHGIGSGAASWLNVAMLLAPQARVITWDAP
GYGASTPLAPAAPSAADYAERLHALLDALDIQSCVLVGHSLGALTAASAARSGSTLASRIRRLVLISPAAGYGAPLRAEA
RAKVRTERLATLDELGIAGMAAKRSGRLVSDTASELARQWVRWNMARLNDGGYRQAVELLCNGDLLADLPPAMPARVACG
TLDVVTTPEACAEVARGCKVTLEPIEGAGHASYVERPEAVAALLREALAA

Nucleotide

• Length: 873 bp

>T144350 NZ_CP061914:1474395-1474793 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin

Protein

• Length: 279 a.a.
• Molecular weight: 30804.14 Da
• Isoelectric Point: 5.4652

>AT144350 WP_206178719.1 NZ_CP046508:2849027-2849863 [Variovorax sp. RKNM96]
MATNDNEAMEEGADRYNVPALERGLRVLCEFSRENRTLSAPELARRFDLPRSTVFRLLTTLENMGFLERAEGGRDYRLGL
AVLRLGFEYLASLELTQLGTPLLNRLCDELRTPCNLVVRDGRSIVYVAKVAPPTPFASSVTVGTRLPAHATVLGRVLLED
LTLPQLRALYPEDKLETFSPSTPKTVNELFDMVQADRSRGYVLGEGFFESNISTIAAPVRDHSGHIVAALGATITSGHIE
ENRMDEMVLRVRSTAEEISSLLNYSPARDNKVVPLRSA

Nucleotide

• Length: 837 bp

>AT144350 NZ_CP061914:1474168-1474395 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGATGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA