Detailed information of TA system TA144349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 3500393..3501046
• Replicon: chromosome
• Accession: NZ_CP046507
• Organism: Mycobacterium avium subsp. avium strain DSM 44156

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: MAA44156_RS16135
• Protein ID: WP_009975163.1
• Coordinates: 3500393..3500854 (-)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: Q742J1
• Locus tag: MAA44156_RS16140
• Protein ID: WP_003872881.1
• Coordinates: 3500858..3501046 (-)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MAA44156_RS16120	3496885..3497271	-	387	WP_009975165.1	hypothetical protein	-
MAA44156_RS16125	3497311..3498894	-	1584	WP_003872878.1	DUF4185 domain-containing protein	-
MAA44156_RS16130	3499890..3500324	-	435	WP_023880080.1	hypothetical protein	-
MAA44156_RS16135	3500393..3500854	-	462	WP_009975163.1	SRPBCC family protein	Toxin
MAA44156_RS16140	3500858..3501046	-	189	WP_003872881.1	antitoxin	Antitoxin
MAA44156_RS16145	3501108..3503480	-	2373	WP_009975162.1	cation-translocating P-type ATPase	-
MAA44156_RS16150	3503477..3505063	-	1587	WP_009975161.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (2-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-51)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16627.13 Da
• Isoelectric Point: 9.5654

>T144349 WP_009975163.1 NZ_CP046507:c3500854-3500393 [Mycobacterium avium subsp. avium]
VAKLSGSIDVPLPPEVAWQHASDLSRYKEWLTIHRVWRSTLPDQNDKGTVVESIVEVKGMLNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKVQPKADGSVVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFARSDPSTNGHR

Nucleotide

• Length: 462 bp

>T144349 NZ_CP061914:1143443-1143778 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6745.42 Da
• Isoelectric Point: 4.4865

>AT144349 WP_003872881.1 NZ_CP046507:c3501046-3500858 [Mycobacterium avium subsp. avium]
MGFLDKAKDLLSQNADKVEMAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKATGATETGDQQG

Nucleotide

• Length: 189 bp

>AT144349 NZ_CP061914:1143195-1143443 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E2WC18