Detailed information of TA system TA144341

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3631..4124
• Replicon: plasmid pIXO134
• Accession: NZ_CP046492
• Organism: Xanthomonas oryzae pv. oryzae strain IXO134

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IXO134_RS23540
• Protein ID: WP_015462614.1
• Coordinates: 3798..4124 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: IXO134_RS23535
• Protein ID: WP_017157755.1
• Coordinates: 3631..3801 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO134_RS23515 (IXO134_023625)	527..1549	+	1023	WP_075244575.1	P-type DNA transfer ATPase VirB11	-
IXO134_RS23520 (IXO134_023630)	1560..2438	+	879	WP_075244574.1	lytic transglycosylase domain-containing protein	-
IXO134_RS23525 (IXO134_023635)	2563..2982	+	420	WP_075244573.1	hypothetical protein	-
IXO134_RS23530 (IXO134_023640)	3059..3445	+	387	WP_075244572.1	hypothetical protein	-
IXO134_RS23535 (IXO134_023645)	3631..3801	+	171	WP_017157755.1	antitoxin MazE family protein	Antitoxin
IXO134_RS23540 (IXO134_023650)	3798..4124	+	327	WP_015462614.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IXO134_RS23545 (IXO134_023655)	4193..7168	-	2976	WP_075244571.1	MobF family relaxase	-
IXO134_RS23550 (IXO134_023660)	7182..8756	-	1575	WP_039587421.1	type IV secretion system DNA-binding domain-containing protein	-
IXO134_RS23555	8760..8933	-	174	Protein_9	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33695	33695

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (2-54)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11676.70 Da
• Isoelectric Point: 9.2550

>T144341 WP_015462614.1 NZ_CP046492:3798-4124 [Xanthomonas oryzae pv. oryzae]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T144341 NZ_CP061913:c168720-168409 [Dactylosporangium vinaceum]
GTGATCGGATCGGAGGCAGCCAAACGCCGGCCTGTGATTGTCGTGAGCAACAACGCCGCCAACCGCAGCGCCGTGTCGCG
AGGACAGGGTGTCGTCACCATCGTCCCCGTTACCAGCAACACCGCCCGCGTGTATCCCTTCCAGGTAAGACTGCCGGCGG
ATCCGGCGATCGGCCTGAACGTCGAGTCCAAGGGCCAAGCCGAACAGGTCCGCTCGATCGACGTCGCACGCATGAGCCAA
CGGCTCGGCACACTCCCGCCGACGTTGATGCGCCAACTGGACCAGGCACTCCGACTGCACCTGGAGCTGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6518.34 Da
• Isoelectric Point: 4.1476

>AT144341 WP_017157755.1 NZ_CP046492:3631-3801 [Xanthomonas oryzae pv. oryzae]
MAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 171 bp

>AT144341 NZ_CP061913:c168971-168756 [Dactylosporangium vinaceum]
ATGAAGGTGAGTGTGAGCCTCCCCGCGGATGACATCGACTTCGTGGACCACTACGCCCGCGAGCGCGGCACGACCCGCTC
AGCCGTCATGCACGAGGCCGTCCAGATGCTTCGCCGCCGCGACCTCGCCATGGACTACGAGGCGGCCAACGACGAATGGG
TGTCCAGCGGCGAGGCCGAGATCTGGAACGCCGTCACCGGTGACGGCCTGCGGTGA