Detailed information of TA system TA144339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3918647..3919392
• Replicon: chromosome
• Accession: NZ_CP046491
• Organism: Xanthomonas oryzae pv. oryzae strain IXO134

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: IXO134_RS18300
• Protein ID: WP_199285672.1
• Coordinates: 3918647..3918976 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: IXO134_RS18305
• Protein ID: WP_237384720.1
• Coordinates: 3918973..3919392 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO134_RS18270 (IXO134_018385)	3913777..3914448	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
IXO134_RS18280 (IXO134_018395)	3914751..3915542	-	792	WP_171970779.1	polysaccharide deacetylase family protein	-
IXO134_RS18285 (IXO134_018400)	3915588..3916514	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
IXO134_RS18290 (IXO134_018405)	3916846..3917181	+	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
IXO134_RS18295 (IXO134_018410)	3917225..3918544	-	1320	WP_263115118.1	IS701-like element ISXo15 family transposase	-
IXO134_RS18300 (IXO134_018415)	3918647..3918976	+	330	WP_199285672.1	DUF4433 domain-containing protein	Toxin
IXO134_RS18305 (IXO134_018420)	3918973..3919392	+	420	WP_237384720.1	macro domain-containing protein	Antitoxin
IXO134_RS18310 (IXO134_018425)	3919444..3920412	+	969	WP_011258529.1	IS5-like element ISXo1 family transposase	-
IXO134_RS18315 (IXO134_018430)	3920612..3921931	+	1320	WP_151421366.1	IS701 family transposase	-
IXO134_RS18320 (IXO134_018435)	3922081..3923049	+	969	WP_011258802.1	IS5-like element ISXo1 family transposase	-
IXO134_RS18325 (IXO134_018440)	3923202..3923840	+	639	WP_011407753.1	phosphate phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3917225..3925113	7888

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13145.86 Da
• Isoelectric Point: 7.1160

>T144339 WP_199285672.1 NZ_CP046491:3918647-3918976 [Xanthomonas oryzae pv. oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T144339 NZ_CP061870:2140507-2140683 [Bacillus subtilis]
GTGCTTGAGAAAGTGGGTATCATAGTTGCTTTCCTCATATCTTTAACGGTTCTTACAATCAACAGTCTAACAATAGTTGA
GAAGATAAGAAACCTAAAGAATGGGACAAGCAAAAAGAAAAAGCGTATACGCAAGCGGCTCCGACCAAAGAGACAACGCC
AACGTATACGCCGATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15621.05 Da
• Isoelectric Point: 6.4733

>AT144339 WP_237384720.1 NZ_CP046491:3918973-3919392 [Xanthomonas oryzae pv. oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQLRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT144339 NZ_CP061870:c2140566-2140466 [Bacillus subtilis]
GATTGTAAGAACCGTTAAAGATATGAGGAAAGCAACTATGATACCCACTTTCTCAAGCACTATGTACACCTCCTTTCCTA
TGACTCTATTATAACATAATT