Detailed information of TA system TA144334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3854402..3855147
• Replicon: chromosome
• Accession: NZ_CP046488
• Organism: Xanthomonas oryzae pv. oryzae strain IXO35

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: IXO35_RS17990
• Protein ID: WP_205420989.1
• Coordinates: 3854402..3854731 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: IXO35_RS17995
• Protein ID: WP_237384720.1
• Coordinates: 3854728..3855147 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO35_RS17960 (IXO35_018045)	3849532..3850203	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
IXO35_RS17970 (IXO35_018055)	3850506..3851297	-	792	WP_171970779.1	polysaccharide deacetylase family protein	-
IXO35_RS17975 (IXO35_018060)	3851343..3852269	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
IXO35_RS17980 (IXO35_018065)	3852601..3852936	+	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
IXO35_RS17985 (IXO35_018070)	3852980..3854299	-	1320	WP_109182077.1	IS701-like element ISXo15 family transposase	-
IXO35_RS17990 (IXO35_018075)	3854402..3854731	+	330	WP_205420989.1	DUF4433 domain-containing protein	Toxin
IXO35_RS17995 (IXO35_018080)	3854728..3855147	+	420	WP_237384720.1	macro domain-containing protein	Antitoxin
IXO35_RS18000 (IXO35_018085)	3855199..3856167	+	969	WP_011258529.1	IS5-like element ISXo1 family transposase	-
IXO35_RS18005 (IXO35_018090)	3856359..3857327	+	969	WP_011258188.1	IS5-like element ISXo1 family transposase	-
IXO35_RS18010 (IXO35_018095)	3857462..3858118	+	657	WP_075246166.1	phosphate phosphatase	-
IXO35_RS18015 (IXO35_018100)	3858156..3859391	-	1236	WP_011257570.1	ISL3-like element ISXoo13 family transposase	-
IXO35_RS18020 (IXO35_018105)	3859693..3860019	+	327	Protein_3451	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3852980..3859391	6411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13159.89 Da
• Isoelectric Point: 7.1161

>T144334 WP_205420989.1 NZ_CP046488:3854402-3854731 [Xanthomonas oryzae pv. oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSELSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T144334 NZ_CP061853:c1824024-1823773 [Bacillus amyloliquefaciens]
ATGAGTTCGCAAGAAATAATCAAGCTAATTGAAAAAGATGGTTGGTACGAAGTCCGAGCAAAAGGAAGTCATCATCAATT
CAAACATCCGACAAAGCCCGGAACGGTTACCATCCCACATCCTAAAAAAGACTTTCCAAAGAAAACATTGAAATCAATCC
TTGAGGGCGGGGCTAAAATAGCCCTCCTTGCAAAAGGATATATAAATATAAATAATAAGAATAAAAATAAAATAAAGGGG
TTAATAAATTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15621.05 Da
• Isoelectric Point: 6.4733

>AT144334 WP_237384720.1 NZ_CP046488:3854728-3855147 [Xanthomonas oryzae pv. oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQLRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT144334 NZ_CP061853:c1823776-1823360 [Bacillus amyloliquefaciens]
TTGAAGAAAGATCGTTACATTTATCCTGCTCTTTTCGATTATGATGACGACGGCATCACTGTAACATTTCCAGATTTACC
TGGGTGTATCACTTTTGGCAACTCCGACGAAGAAGCTTTAGCAATGGCAAAAGAAGCTATGGCCTTGCACCTTTATGGCT
TCGAACAAGACGGCGATATAATACCTGAAGCTACACCTTCAAGGGAAATCAAAGCAGAGGAAAGCCAAAGCATCGTTTTA
ATTGAAACTTGGATGCCCCCATTTCGTCATGATATGGAAAATGCAGCAGTTAAGAAAACACTTACTATCCCCCGCTGGAT
GGATGACATCGCAAAAGAGCATAAAGTGAATTATTCACAGTTGCTTCAAGAGGCCATTAAAGAACATCTTGGGATTTACA
AAAATCCAAGGTCTTAA