Detailed information of TA system TA144291

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 4172718..4173361
• Replicon: chromosome
• Accession: NZ_CP046445
• Organism: Leclercia sp. 119287

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: GNG27_RS20010
• Protein ID: WP_156264668.1
• Coordinates: 4172945..4173361 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A345CHX5
• Locus tag: GNG27_RS20005
• Protein ID: WP_039032651.1
• Coordinates: 4172718..4172948 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNG27_RS19990	4169205..4170148	+	944	Protein_3916	IS3 family transposase	-
GNG27_RS19995	4170494..4170796	-	303	WP_185902359.1	hypothetical protein	-
GNG27_RS20000	4171031..4172296	+	1266	WP_142489622.1	NO-inducible flavohemoprotein	-
GNG27_RS20005	4172718..4172948	+	231	WP_039032651.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GNG27_RS20010	4172945..4173361	+	417	WP_156264668.1	type II toxin-antitoxin system VapC family toxin	Toxin
GNG27_RS20015	4173385..4174128	+	744	WP_156264669.1	hypothetical protein	-
GNG27_RS20020	4174384..4175078	-	695	Protein_3922	IS1 family transposase	-
GNG27_RS20025	4175169..4175738	+	570	WP_156264670.1	type 1 fimbrial protein	-
GNG27_RS20030	4175797..4176492	+	696	WP_105902572.1	molecular chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	4169205..4175078	5873

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15078.44 Da
• Isoelectric Point: 6.4552

>T144291 WP_156264668.1 NZ_CP046445:4172945-4173361 [Leclercia sp. 119287]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRNQRIVISAITYSEMRFGATGPKSSPRHIELVDAFCARLDAILPWDSA
AVDATTDIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLTIEDWVK

Nucleotide

• Length: 417 bp

>T144291 NZ_CP061839:1809298-1809729 [Treponema pedis]
ATGTATTTGCTAGATACAAATATCTGTATTTTCCTCATAAAGAATAAAAATCTACATTTAAAGAAAAAAATATTTAACTG
TAAAAAAGAAGAATTATTTCTTTCTTCCATTACAATTGCTGAGTTGGAGTATGGTGTTGCAAAAAGTCAATTTAGAGAAA
AGAATCGCCATGCTCTTTTGGATTTTTGCTCCGATTTTACTAACATAATAGGTTTTACGACAGAAGATACAGAAACATAC
GGAATGATTAGAGCTTATCTTGAAAATAAAGGGATTCCGATTGGACCTTTTGATACACAGATTGCTGCTCAAGGTTTAGC
AAGAAATCTTACTGTGGTTACAAATAATATAAAAGAATTTTCTAGAATACCGGGACTAAAAGTTGAAGACTGGACAAAAA
ATAATATAAACCTAACACCCGCTTCAACCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8737.83 Da
• Isoelectric Point: 4.3654

>AT144291 WP_039032651.1 NZ_CP046445:4172718-4172948 [Leclercia sp. 119287]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFDL

Nucleotide

• Length: 231 bp

>AT144291 NZ_CP061839:1809068-1809298 [Treponema pedis]
ATGGTTTGTGCAAAAGTATTCACAAGCGGAAATAGTCAGGCTGTAAGGATACCTAAAGAATTTCATATTGATTTTTCTGA
ATTATTTATCAAGAAAATCGGTTCATCAATAATCCTAACACCAAAAGAAAGCAATTGGGAAAATCTTGAAAGAAGTCTTT
CTGAGTTTTCCGATGATTTCATGACAAAAGGAAGAAGTCAACCGGCAACGCAGGAAAGGGAAGTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A345CHX5