Detailed information of TA system TA144288

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3098886..3099476
• Replicon: chromosome
• Accession: NZ_CP046445
• Organism: Leclercia sp. 119287

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: GNG27_RS15035
• Protein ID: WP_142486330.1
• Coordinates: 3098886..3099218 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: GNG27_RS15040
• Protein ID: WP_142486331.1
• Coordinates: 3099219..3099476 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNG27_RS15005	3093955..3095028	+	1074	WP_156264162.1	porin	-
GNG27_RS15010	3095161..3096774	+	1614	WP_156264163.1	sensor histidine kinase	-
GNG27_RS15015	3096771..3097496	+	726	WP_156264164.1	two-component system response regulator DcuR	-
GNG27_RS15025	3097843..3098640	+	798	WP_156264165.1	DgsA anti-repressor MtfA	-
GNG27_RS15035	3098886..3099218	-	333	WP_142486330.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GNG27_RS15040	3099219..3099476	-	258	WP_142486331.1	antitoxin	Antitoxin
GNG27_RS15045	3099609..3100559	-	951	WP_142486332.1	HTH-type transcriptional regulator Cbl	-
GNG27_RS15050	3100656..3101573	-	918	WP_142486333.1	nitrogen assimilation transcriptional regulator NAC	-
GNG27_RS15060	3102069..3103904	-	1836	WP_156264166.1	patatin-like phospholipase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

• Antitoxin PemI-like (5-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11813.77 Da
• Isoelectric Point: 10.4752

>T144288 WP_142486330.1 NZ_CP046445:c3099218-3098886 [Leclercia sp. 119287]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKAAFNHLTRLPVVVPVTTGGKFARTAGFAVSLDEAGSKTRGVIRCDQPRTI
DMGARHGIKLERVPDSVMNEVLAKLGALLT

Nucleotide

• Length: 333 bp

>T144288 NZ_CP061832:c5289144-5288761 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9285.71 Da
• Isoelectric Point: 4.2950

>AT144288 WP_142486331.1 NZ_CP046445:c3099476-3099219 [Leclercia sp. 119287]
MYITRLKKVGGSVMLAVPPAILKTLDLQPDSEVGVTIDNGCLIVEPQKRPHYSLAELLAQCDPQAELSEEDREWVDAPAA
GKELL

Nucleotide

• Length: 258 bp

>AT144288 NZ_CP061832:c5289386-5289144 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA