Detailed information of TA system TA144278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 1980231..1980877
• Replicon: chromosome
• Accession: NZ_CP046445
• Organism: Leclercia sp. 119287

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: GNG27_RS09575
• Protein ID: WP_156263521.1
• Coordinates: 1980458..1980877 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A345CHX5
• Locus tag: GNG27_RS09570
• Protein ID: WP_039032651.1
• Coordinates: 1980231..1980461 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNG27_RS09545	1975329..1976357	-	1029	WP_156263519.1	sensor domain-containing diguanylate cyclase	-
GNG27_RS09550	1976478..1976759	+	282	WP_156263520.1	hypothetical protein	-
GNG27_RS09555	1977242..1978321	-	1080	WP_197090577.1	hypothetical protein	-
GNG27_RS09560	1978468..1979588	+	1121	WP_096147877.1	IS3-like element ISSen4 family transposase	-
GNG27_RS09565	1979621..1979803	-	183	Protein_1867	helix-turn-helix domain-containing protein	-
GNG27_RS09570	1980231..1980461	+	231	WP_039032651.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GNG27_RS09575	1980458..1980877	+	420	WP_156263521.1	type II toxin-antitoxin system VapC family toxin	Toxin
GNG27_RS09580	1980916..1981170	+	255	Protein_1870	hypothetical protein	-
GNG27_RS09585	1981339..1981689	-	351	WP_156263522.1	DUF305 domain-containing protein	-
GNG27_RS09590	1981843..1982274	-	432	WP_156263523.1	copper-binding protein	-
GNG27_RS09595	1982514..1983989	-	1476	WP_156263524.1	Cu(+)/Ag(+) sensor histidine kinase	-
GNG27_RS09600	1983979..1984662	-	684	WP_156263525.1	copper/silver response regulator transcription factor SilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15180.59 Da
• Isoelectric Point: 7.8603

>T144278 WP_156263521.1 NZ_CP046445:1980458-1980877 [Leclercia sp. 119287]
VNKTYMLDTNICSFIIREQPEAVLKRLEQAVLRNQRIVVSAITYSEMRFGATGPKAASRHIQLVDAFCARLDAILPWDRT
AVDATTEIKVALRLAGTPIGPNNTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVIA

Nucleotide

• Length: 420 bp

>T144278 NZ_CP061832:200102-200587 [Klebsiella pneumoniae]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAACCAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTACTTCGTTGCTATCGGGTTGCCGAGAATATAGGTGTACGTGCAATCATGGTTCATGCGCTCACAG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8737.83 Da
• Isoelectric Point: 4.3654

>AT144278 WP_039032651.1 NZ_CP046445:1980231-1980461 [Leclercia sp. 119287]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQLEKADPDFMAEREDVVTDEGRFDL

Nucleotide

• Length: 231 bp

>AT144278 NZ_CP061832:199848-200114 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAGCTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A345CHX5