Detailed information of TA system TA144277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 1156222..1156841
• Replicon: chromosome
• Accession: NZ_CP046445
• Organism: Leclercia sp. 119287

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: A0A345CDT0
• Locus tag: GNG27_RS05635
• Protein ID: WP_032616864.1
• Coordinates: 1156222..1156440 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: GNG27_RS05640
• Protein ID: WP_142488463.1
• Coordinates: 1156449..1156841 (-)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNG27_RS05615	1153135..1153488	+	354	WP_142488459.1	DUF1428 family protein	-
GNG27_RS05620	1153521..1155089	-	1569	WP_156263102.1	EAL domain-containing protein	-
GNG27_RS05625	1155283..1155423	+	141	WP_142488461.1	type B 50S ribosomal protein L36	-
GNG27_RS05630	1155483..1155947	-	465	WP_142488462.1	hypothetical protein	-
GNG27_RS05635	1156222..1156440	-	219	WP_032616864.1	hemolysin expression modulator Hha	Toxin
GNG27_RS05640	1156449..1156841	-	393	WP_142488463.1	Hha toxicity modulator TomB	Antitoxin
GNG27_RS05645	1157334..1160474	-	3141	WP_142488464.1	multidrug efflux RND transporter permease subunit AcrB	-
GNG27_RS05650	1160497..1161690	-	1194	WP_142488465.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-115)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8583.95 Da
• Isoelectric Point: 8.9008

>T144277 WP_032616864.1 NZ_CP046445:c1156440-1156222 [Leclercia sp. 119287]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTAVWKFVR

Nucleotide

• Length: 219 bp

>T144277 NZ_CP061830:95332-95688 [Acinetobacter seifertii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGCACAGCAAGATCAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ATATGAAAGACCTACGTGTTCAGCATCGAGGTAAATCACTGAGAGCCTTTTTCGCGTTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGTGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAAAT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 15122.00 Da
• Isoelectric Point: 4.8941

>AT144277 WP_142488463.1 NZ_CP046445:c1156841-1156449 [Leclercia sp. 119287]
MDEYSPKRQDIAQLKFLCESLYHDCLSNLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNASRANPLASPVKIITNN

Nucleotide

• Length: 393 bp

>AT144277 NZ_CP061830:95681-95983 [Acinetobacter seifertii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAATCGCTCTCCAGAGAGTCAAGCTCGTATTCAACAAATGGCGGATGAATT
ATTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAATTGGCTGCAACCCTTGGAATCA
AACAGCCTTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTCGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTCAATGTCTAA

Structures

Toxin

• AlphaFold DB: A0A345CDT0