Detailed information of TA system TA144275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): KacT-ataR/DUF1778(antitoxin)
• Location: 608431..609225
• Replicon: chromosome
• Accession: NZ_CP046445
• Organism: Leclercia sp. 119287

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: GNG27_RS02920
• Protein ID: WP_156265027.1
• Coordinates: 608431..608931 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: GNG27_RS02925
• Protein ID: WP_142488082.1
• Coordinates: 608956..609225 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNG27_RS02895	603597..603851	-	255	WP_156262824.1	DUF4282 domain-containing protein	-
GNG27_RS02900	603966..605357	-	1392	WP_156262825.1	PLP-dependent aminotransferase family protein	-
GNG27_RS02905	605458..605937	+	480	WP_142488079.1	carboxymuconolactone decarboxylase family protein	-
GNG27_RS02910	605924..606826	-	903	WP_156262826.1	LysR family transcriptional regulator	-
GNG27_RS02915	606927..608342	+	1416	WP_156262827.1	aldehyde dehydrogenase family protein	-
GNG27_RS02920	608431..608931	-	501	WP_156265027.1	GNAT family N-acetyltransferase	Toxin
GNG27_RS02925	608956..609225	-	270	WP_142488082.1	DUF1778 domain-containing protein	Antitoxin
GNG27_RS02930	609388..609711	+	324	WP_156262828.1	antibiotic biosynthesis monooxygenase	-
GNG27_RS02935	609704..610099	+	396	WP_142488084.1	amino acid-binding protein	-
GNG27_RS02940	610074..610769	+	696	WP_156262829.1	winged helix-turn-helix domain-containing protein	-
GNG27_RS02945	611065..611454	+	390	WP_156262830.1	hypothetical protein	-
GNG27_RS02950	611761..612324	+	564	WP_185902332.1	helix-turn-helix transcriptional regulator	-
GNG27_RS02955	612342..612683	+	342	WP_156262831.1	hypothetical protein	-
GNG27_RS02960	612799..613266	+	468	WP_156262832.1	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (78-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18499.30 Da
• Isoelectric Point: 7.3712

>T144275 WP_156265027.1 NZ_CP046445:c608931-608431 [Leclercia sp. 119287]
MFSQTTHCDLTGFNCGEDTLNRFLCEHLARQHVGRLLRAYLLVTDDPTPKVLGYYTLSGSCFEKETLPSKTQQRKVPYVN
VPSVTLGRLAIHQDLQGQGWGMTLVTHAMKVVWLASQAVGIHGMFVDALNDKAKDFYLTLGFIPLTGSNADALFYPTKSI
EKLFAE

Nucleotide

• Length: 501 bp

>T144275 NZ_CP061819:c49633-49079 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTC
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGCG
AAACTTTATGAGAAAGACGTAGTAAAACACGTCACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGCTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTTATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGGTTCTGTTGCAAAGTTTTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10415.85 Da
• Isoelectric Point: 5.2171

>AT144275 WP_142488082.1 NZ_CP046445:c609225-608956 [Leclercia sp. 119287]
MPALKKQRIDLRLTDEDKDLIEEAAAMTNQTITQFMVNSASERAAEVIEQHRRLILNEDSWKRVMDALDNPPEPNERLKR
AARRLQNME

Nucleotide

• Length: 270 bp

>AT144275 NZ_CP061819:c49907-49635 [Enterococcus faecium]
ATGGCAGTTACATATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA