Detailed information of TA system TA144274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 587855..588891
• Replicon: chromosome
• Accession: NZ_CP046445
• Organism: Leclercia sp. 119287

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: GNG27_RS02810
• Protein ID: WP_142488063.1
• Coordinates: 587855..588430 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: GNG27_RS02815
• Protein ID: WP_106995644.1
• Coordinates: 588430..588891 (-)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNG27_RS02805	586967..587566	-	600	WP_156262810.1	response regulator transcription factor	-
GNG27_RS02810	587855..588430	-	576	WP_142488063.1	PIN domain-containing protein	Toxin
GNG27_RS02815	588430..588891	-	462	WP_106995644.1	helix-turn-helix domain-containing protein	Antitoxin
GNG27_RS02820	589047..589433	-	387	WP_156262811.1	VOC family protein	-
GNG27_RS02825	589435..590592	-	1158	WP_156262812.1	MFS transporter	-
GNG27_RS02830	590589..590747	-	159	WP_156262813.1	hypothetical protein	-
GNG27_RS02835	590846..591616	+	771	WP_156262814.1	DeoR/GlpR family DNA-binding transcription regulator	-
GNG27_RS02840	591613..591909	-	297	WP_156262815.1	SH3 domain-containing protein	-
GNG27_RS02845	592116..593237	+	1122	WP_156262816.1	cupin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-114)

Antitoxin

• Helix-turn-helix domain, group 17 (84-132)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21747.96 Da
• Isoelectric Point: 4.8643

>T144274 WP_142488063.1 NZ_CP046445:c588430-587855 [Leclercia sp. 119287]
MTHSPYPVVLDACVLYPSMLRDLLMHIGKTGLYQPKWTTQIHDEWQRNLLINRPDITADQLKRTEMLMNKAFGDACVSGY
ESIIDGLSLPDQDDRHVLAAAIRSDSEVIVTYNLVDFPGGNLAEFDVEALHPDEFLSDLFDLNHALVLEAVRMQRSHLRH
PPLNPDQFLQGLLQLGLPKTVTELEKYRVMI

Nucleotide

• Length: 576 bp

>T144274 NZ_CP061818:c157524-156661 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTC
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGCG
AAACTTTATGAGAAAGACGTAGTAAAACACGTCACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGCTATAATTTAGTGATTGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTTATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACTATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GATTAGAAACGCCTTCTATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGCTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGACTTTAA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 17085.59 Da
• Isoelectric Point: 6.3368

>AT144274 WP_106995644.1 NZ_CP046445:c588891-588430 [Leclercia sp. 119287]
MTNSLLNSLTLPAPKEIEAAVRGQRELATFLSTKQETQKITIQDAQDVTHQIELPTSSLTLLMSILGELAAGNAVQVVPV
HAELTTQEAANILNVSRPHMVKLLEEGKLPFHKTGRHRRILFADLMKYKDQRDAESIQALRELAEQSQELGFY

Nucleotide

• Length: 462 bp

>AT144274 NZ_CP061818:c157798-157526 [Enterococcus faecium]
ATGGCAGTTACATATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA