Detailed information of TA system TA144271

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rlegTA/AbiEii-AbiEi
• Location: 3759135..3760633
• Replicon: chromosome
• Accession: NZ_CP046444
• Organism: Granulicella sp. 5B5

Toxin (Protein)

• Gene name: rlegT
• Uniprot ID: -
• Locus tag: GOB94_RS15905
• Protein ID: WP_182276817.1
• Coordinates: 3759716..3760633 (+)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: rlegA
• Uniprot ID: -
• Locus tag: GOB94_RS15900
• Protein ID: WP_182278676.1
• Coordinates: 3759135..3759719 (+)
• Length: 195 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GOB94_RS15890	3754295..3756307	+	2013	WP_182276815.1	type IV secretion system DNA-binding domain-containing protein	-
GOB94_RS15895	3756314..3759064	+	2751	WP_182276816.1	relaxase domain-containing protein	-
GOB94_RS15900	3759135..3759719	+	585	WP_182278676.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
GOB94_RS15905	3759716..3760633	+	918	WP_182276817.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GOB94_RS15910	3760845..3762668	+	1824	WP_182276818.1	DUF2130 domain-containing protein	-
GOB94_RS15915	3763449..3763751	+	303	WP_182278677.1	ATP-binding protein	-
GOB94_RS15920	3763679..3764062	+	384	WP_182278698.1	hypothetical protein	-
GOB94_RS15925	3764171..3764386	+	216	WP_182276819.1	hypothetical protein	-
GOB94_RS15930	3764422..3764583	+	162	WP_182276820.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	3744139..3762945	18806

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (39-266)

Antitoxin

• Transcriptional regulator, AbiEi antitoxin (11-49)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34384.29 Da
• Isoelectric Point: 5.7800

>T144271 WP_182276817.1 NZ_CP046444:3759716-3760633 [Granulicella sp. 5B5]
MTREPNKNPAASIRARLLSLSREKGEDYQRVLGRFAIERFLYRLGISTYRDRFVLKGATLFTLWTGETHRPTKDLDLLGW
GSSAIADVEQVFRDICEITEDDGIVFDSQSVEGTTIKENDEYNGVHVRFRAQLAGAQMSLQVDIGFGDAVFPEPELATFP
VLLPMSAPIIRAYPREASIAEKLHAMVQLDIRNSRMKDFYDIWFMANTWTFRLESLRNAIALSFQRRGSVMPNGVPFALT
DEFLTDAQKLQLWKAFAGRLNVGDKALTLAEVGAVLRAFLVPCLSSKSDIAQVWTPTIGWHESES

Nucleotide

• Length: 918 bp

>T144271 NZ_CP061816:1019993-1020238 [Brucella pinnipedialis]
ATGAAGATCATCTGGGACGAACCGAAGCGACAGACCAACATTGCCAAGCACGGCTTGGACTTCGCTGACCTGCATTTCGA
ATTCTTCCTGTCGGCTAAGGTCTTCCCCACCAAGGCAGATCGCCTGATGGCAATCGGAGAATTCAACGGCCTGATTATCA
TCGCCGTCATTTTCAAGCCGGTTGGTTCGGAAGCCCTCTCCGTGATCTCCATGCGTCCAGCAAGCCAGAAGGAAAGGAAG
CTCTGA

Antitoxin

Protein

• Length: 195 a.a.
• Molecular weight: 21680.34 Da
• Isoelectric Point: 10.5047

>AT144271 WP_182278676.1 NZ_CP046444:3759135-3759719 [Granulicella sp. 5B5]
MVKTIRYVGKHGIVRPRDLEALGIPREYLLRLYRRGKLSRTGRGVYTLPDAAITERHSYAEVTKRVPGAVLCLLTALAFH
EITTQNPSSIWIALGKGARTPVLESPSLRIARLSGPSLTEGIETHRVEGISVRVYSAAKTVADCFKFRNKIGLDVAIEAL
RDCLSQRKASINDIYRYAKICRVSNVIRPYMEAL

Nucleotide

• Length: 585 bp

>AT144271 NZ_CP061816:1020238-1020552 [Brucella pinnipedialis]
ATGGGTATCAAATTTTCCTCTAAGCGCCCTCTCACTCAGGAAGAAGAGGCAGAAATTCAGAAGATGATCGCAAGCGATCC
CGATGCACCGGAAGCCACCGATGAACAGCTTGCCAAGGCCAAGCCTTTCAAGGAAGCCTTTCCAGATATGGCCGCCAAGA
TGGAGAAGGCCATTCGAGGCTGGCCGCGCATCGACAATCCCAAGACGCCGGTTACTATCCGGCTCGATCAGGATGTAGTT
CAACGCTTCAAGGCCACCGGCAAAGGCTGGCAGGGCCGTATGAACGATGCCCTGCGCAAAGCTGTCGGGCTTTGA