Detailed information of TA system TA144262

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 65693..66366
• Replicon: plasmid unnamed1
• Accession: NZ_CP046442
• Organism: Pseudomonas coronafaciens pv. coronafaciens strain B19001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q52562
• Locus tag: GMO17_RS26910
• Protein ID: WP_005769733.1
• Coordinates: 65693..66112 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GMO17_RS26915
• Protein ID: WP_024671647.1
• Coordinates: 66109..66366 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GMO17_RS26890	62204..62860	+	657	WP_157973363.1	DUF2971 domain-containing protein	-
GMO17_RS26895	62955..63560	-	606	WP_080268302.1	histidine phosphatase family protein	-
GMO17_RS26900	63800..64735	-	936	WP_024671645.1	syringolide biosynthetic protein AvrD1	-
GMO17_RS26905	65020..65652	-	633	WP_024671646.1	recombinase family protein	-
GMO17_RS26910	65693..66112	-	420	WP_005769733.1	type II toxin-antitoxin system VapC family toxin	Toxin
GMO17_RS26915	66109..66366	-	258	WP_024671647.1	Arc family DNA-binding protein	Antitoxin
GMO17_RS26920	66716..67372	+	657	WP_017684244.1	hypothetical protein	-
GMO17_RS26925	67420..67674	+	255	WP_024671648.1	hypothetical protein	-
GMO17_RS26930	68059..68598	+	540	WP_024671649.1	hypothetical protein	-
GMO17_RS26935	68858..69499	+	642	WP_191867948.1	SOS response-associated peptidase	-
GMO17_RS26940	69874..70122	+	249	WP_055004694.1	hypothetical protein	-
GMO17_RS26945	70169..70441	-	273	WP_147458958.1	hypothetical protein	-
GMO17_RS26950	70490..70723	+	234	WP_024669557.1	hypothetical protein	-
GMO17_RS26955	70736..70987	+	252	WP_019331343.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..112911	112911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Arc-like DNA binding domain (5-46)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15138.43 Da
• Isoelectric Point: 4.7010

>T144262 WP_005769733.1 NZ_CP046442:c66112-65693 [Pseudomonas coronafaciens pv. coronafaciens]
MILLDTNVISEPQRREPNAHVLDWIDAQALETLYLSTITVAELRAGIALMPVGKRQDSLRENLEKHLLPMFANRVLSFDM
TCTKAYAELLAKSRAAGLAVETADAFIAAIALANGFTVATRDTGPFEAAGLNVINPWEA

Nucleotide

• Length: 420 bp

>T144262 NZ_CP061801:1762143-1762691 [Enterobacter mori]
GTGGCCAATCTCACGATCGATATGTTTTCAGAGGGAACCGATTACACTTTCGAGGATTTCGATTGTGGTGAGCCCTCACT
CAATTCCTTCCTCACAGAGCATCTGGTGCGTCAGCACAATGGGCGTATTTTAAGAGGCTATCTACTCAAAGATCGTGACC
ATCCTCGTGTGCTGGGGTACTACACGTTGTCCGGAAGTTGTTTTGAGAAAGCATTGTTACCCTCTAAAACCCAGCAGCGC
AGAATTCCTTATACAAACGTCCCAGGTGTGACTTTAGGCCGTTTAGCGGTACATAAAGAATTGCAGGGGAATGAGTGGGG
GACAACACTCGTCACGCACGCTATGCGGGTTGTCTACCTGGCATCGCAGGCTGTGGGCGTGCATGGGATTTTTGTTGATG
CACTTAATGAGCAGGCAAAAAGATTTTATCTGAAGCAAGGTTTTATCCCGTTATCCGGTGAAAACAGCAATTCGCTTTTT
TATCCCACGAAATCAATCGAACGATTGTTTGAAGCAGACGAGTACAGCCCTCCCGGAGAAGGGCTTTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9415.65 Da
• Isoelectric Point: 9.0330

>AT144262 WP_024671647.1 NZ_CP046442:c66366-66109 [Pseudomonas coronafaciens pv. coronafaciens]
VANVNVRNLPDEVHRAIRIQAALHGRSTEAEIRDILERAARPEGRVKLGTFLASIAREVGGLTDKEHTLFENTRITSPAR
AVSFE

Nucleotide

• Length: 258 bp

>AT144262 NZ_CP061801:1761870-1762139 [Enterobacter mori]
ATGCCAGCATTGAAAAAACAGCGTATCGACCTTCGCTTAACTGATGAAGACAAAACGATGATCGAAGAAGCGGCGGCGAT
GACGAATCAAACGATCACCCAATTTATGGTGAACAGCGCTTCGGAACGTGCTGCAGAAGTGATAGAACAGCATCGACGTC
TGGTGTTAAACGAAGCATCATGGAATGCAGTGATGGATGCCATAAACAATCCGCCAGAGCCGAATGAGAGACTAAAACGA
GCGGCAAAGCGTCTTCAAAACATGGAGTGA

Structures

Toxin

• AlphaFold DB: Q52562