Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 11953..12545 Replicon plasmid pS4-mcr1.1
Accession NZ_CP046418
Organism Escherichia coli strain LZYWN_4

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag GJV83_RS23645 Protein ID WP_000520549.1
Coordinates 11953..12201 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag GJV83_RS23650 Protein ID WP_000681613.1
Coordinates 12198..12545 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJV83_RS23615 8830..9345 + 516 WP_001025395.1 J domain-containing protein -
GJV83_RS23620 9450..9992 + 543 WP_001083906.1 hypothetical protein -
GJV83_RS23625 9989..10240 + 252 WP_001230707.1 hypothetical protein -
GJV83_RS23630 10227..10454 + 228 WP_000801440.1 hypothetical protein -
GJV83_RS23635 10908..11207 + 300 WP_000650309.1 hypothetical protein -
GJV83_RS23640 11298..11648 + 351 WP_000854259.1 hypothetical protein -
GJV83_RS23645 11953..12201 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
GJV83_RS23650 12198..12545 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GJV83_RS23655 12906..13409 + 504 WP_000781813.1 hypothetical protein -
GJV83_RS23660 13413..14642 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
GJV83_RS23665 14719..15012 + 294 WP_060598542.1 LuxR family transcriptional regulator -
GJV83_RS23670 15009..15224 + 216 WP_000760375.1 EexN family lipoprotein -
GJV83_RS23675 15580..16200 + 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
GJV83_RS23680 16268..16540 + 273 WP_001328551.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..34645 34645


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T144260 WP_000520549.1 NZ_CP046418:11953-12201 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T144260 NZ_CP061801:c1143047-1142829 [Enterobacter mori]
ATGTCTGATAAACCATTAACCAAGATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATAGACACGCTGGAACGTGT
AATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCTGTATTTTATTCAGCGGCTGACCATCGTCTTGCTGAGC
TAACAATGAATAAACTGTATGACAAAATCCCCTCTTCTGTGTGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT144260 WP_000681613.1 NZ_CP046418:12198-12545 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT144260 NZ_CP061801:c1143449-1143075 [Enterobacter mori]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGATGAAAGTAACCACGGCTGGGTGAACGATCCTACGTCTGCTATCAATTTACAGCTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTGATTGAGCAAATTGACGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAAAAATGGCGTAAATCTGGGAATCGTCT
ATTTCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCTGTTAGCCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References