Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 9989..10454 Replicon plasmid pS4-mcr1.1
Accession NZ_CP046418
Organism Escherichia coli strain LZYWN_4

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag GJV83_RS23625 Protein ID WP_001230707.1
Coordinates 9989..10240 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag GJV83_RS23630 Protein ID WP_000801440.1
Coordinates 10227..10454 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJV83_RS23600 5470..5997 - 528 WP_000577043.1 DUF2726 domain-containing protein -
GJV83_RS23605 6132..6536 - 405 WP_012421203.1 hypothetical protein -
GJV83_RS23610 6549..7415 - 867 WP_001353743.1 replication initiation protein -
GJV83_RS23615 8830..9345 + 516 WP_001025395.1 J domain-containing protein -
GJV83_RS23620 9450..9992 + 543 WP_001083906.1 hypothetical protein -
GJV83_RS23625 9989..10240 + 252 WP_001230707.1 hypothetical protein Toxin
GJV83_RS23630 10227..10454 + 228 WP_000801440.1 hypothetical protein Antitoxin
GJV83_RS23635 10908..11207 + 300 WP_000650309.1 hypothetical protein -
GJV83_RS23640 11298..11648 + 351 WP_000854259.1 hypothetical protein -
GJV83_RS23645 11953..12201 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
GJV83_RS23650 12198..12545 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
GJV83_RS23655 12906..13409 + 504 WP_000781813.1 hypothetical protein -
GJV83_RS23660 13413..14642 + 1230 WP_001329504.1 relaxase/mobilization nuclease domain-containing protein -
GJV83_RS23665 14719..15012 + 294 WP_060598542.1 LuxR family transcriptional regulator -
GJV83_RS23670 15009..15224 + 216 WP_000760375.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..34645 34645


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-69)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T144259 WP_001230707.1 NZ_CP046418:9989-10240 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T144259 NZ_CP061801:543690-543974 [Enterobacter mori]
ATGACTTATGAGTTGGAATTCGACCCTGGAGCGCTAAAGGAGTGGCATAAACTGGGCGATACCGTGAAAACCCAATTTAA
GAAGAAATTGGTCGATGTACTGGTGGATCCCAGGATCGAATCCGCCCGTTTACATGGGCTGCATGATTGCTACAAAATTA
AGCTTAAATCATCCGGCTACCGTTTGGTCTATCAGGTGCGGGATGAGGTTGTGATGGTGTTTGTTGTTGCCATAGGCAAA
CGAGAGAAATCAGCCGTTTATCATGATGCAGATAAACGGCTATAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT144259 WP_000801440.1 NZ_CP046418:10227-10454 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT144259 NZ_CP061801:543458-543700 [Enterobacter mori]
ATGGCCACGCTGAACGTCCGTCTGGACGACAAACTTAAAAATGAGGCTTATGCAGTGCTGGAAAAACTCAATATCACCCC
AACCGAAGCTGTAAGATTACTGTTTCAGTACGTCGCAGAGACGGGGCGTATGCCGGTGAAAACGGTGACCATTAGCGACG
GTGAAGACGCATTGATTCAGACAGTCCGGGAGCGACTGGCTAATCCACAAAAAGGAATTAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References