Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 104408..105051 Replicon plasmid pS4-aph
Accession NZ_CP046417
Organism Escherichia coli strain LZYWN_4

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag GJV83_RS23530 Protein ID WP_001044768.1
Coordinates 104635..105051 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag GJV83_RS23525 Protein ID WP_001261287.1
Coordinates 104408..104638 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJV83_RS23515 99588..100721 - 1134 WP_000545983.1 DUF3800 domain-containing protein -
GJV83_RS23520 100984..104102 - 3119 Protein_125 hypothetical protein -
GJV83_RS23525 104408..104638 + 231 WP_001261287.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
GJV83_RS23530 104635..105051 + 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
GJV83_RS23535 105213..107351 - 2139 WP_039002805.1 AAA family ATPase -
GJV83_RS23540 107816..108811 + 996 WP_000246636.1 hypothetical protein -
GJV83_RS23545 108815..109747 + 933 WP_000991832.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(M) / sul3 / aph(4)-Ia / aac(3)-IVa / blaTEM-1B - 1..113995 113995


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T144258 WP_001044768.1 NZ_CP046417:104635-105051 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T144258 NZ_CP061801:c342971-342594 [Enterobacter mori]
ATGCAAACACAACCTGTTCCTCCGAAACGGGAGGTTTCGCCACGCCCGTCACCCGTGGCGATCTGGCAGCGCCTCCTGAG
TCACCTGCTGGACCGTCACTACGGTCTGACGCTCAACGACACGCCATTTGGCAAAGATGGTGTGATCCAAGAGCATATCG
ATGCCGGTATCTCACTGTGCGACGCGGTGAATTTCATCGTGGAGAAATACGACCTGGTACGCACTGATCGTCGCGGTTTT
AACGCGGAAACTCAGTCGCCGCTTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAGCCACCGGGCTGATGACCCGTCA
TAGCTACAGGGCCGTTACCGACATCACCACCGGCAAATACCGTGAGGTGCAACCATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT144258 WP_001261287.1 NZ_CP046417:104408-104638 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT144258 NZ_CP061801:c343347-343021 [Enterobacter mori]
ATCGCCGCCCCTTGGTGGGGGCTTAAACGCGATATCACCCCCTGTTTTGGCGCACGTCTGGTGCAGGAGGGCAACCGGCT
GCATTACCTGAACGACCGCGCCAGCATCACCGGCACCTTCAGCGACGCGGATTTACGCCATCTCGACCAGGCATTCCCGC
TACTGCTGAAGCAACTGGAGCTGATGCTGCTTTCCGGTGAGCTGAATCCCCGTCATCAGCACTGCGTCACGCTGTATGCA
AAAGGGCTGATATGCGAGGCCGACAGCCTCGGCTCGCACGGGTACGTCTATCTCGCAATTTACCCGACACCTGCAACAAC
AGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References