Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4254374..4254871 Replicon chromosome
Accession NZ_CP046416
Organism Escherichia coli strain LZYWN_4

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GJV83_RS20690 Protein ID WP_001173343.1
Coordinates 4254374..4254547 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GJV83_RS20695 Protein ID WP_000398619.1
Coordinates 4254575..4254871 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJV83_RS20680 4251044..4252501 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
GJV83_RS20685 4252738..4254255 + 1518 WP_001295074.1 lysine--tRNA ligase -
GJV83_RS20690 4254374..4254547 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GJV83_RS20695 4254575..4254871 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GJV83_RS20700 4255098..4255370 - 273 WP_000405651.1 N-acetyltransferase -
GJV83_RS20705 4255382..4255612 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
GJV83_RS20710 4255793..4257424 + 1632 WP_001216477.1 sensor histidine kinase -
GJV83_RS20715 4257421..4258140 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T144249 WP_001173343.1 NZ_CP046416:c4254547-4254374 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T144249 NZ_CP061800:3066217-3066486 [Desulfonema magnum]
ATGAATGCAATAGCTTATTCAAATTTCAGAAAAAATTTTGCTAATATAATGGAAAAAATATGTGAAGATCACGATCCGGT
AATTATTACTCGTAAAAATTTAAAACCGGTTGTGATGATGTCTCTTGAGGACTACAATGCCATTGAAGAAACAATGTATT
TATTACGCAGTCCTGCCAATGCCGCCCAATTACGTGCCAGCATTGCCGAATTTGAAGCCGGTAATTTTAACATACGGAAA
ATGATTGATAATGATGATTGCATGGACTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT144249 WP_000398619.1 NZ_CP046416:c4254871-4254575 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT144249 NZ_CP061800:3066467-3066721 [Desulfonema magnum]
ATGATGATTGCATGGACTGACCGGGCTTGGAACGAATACTTGTACTGGCAAAAACATGACAAAAAAATTGTTAAACGGAT
CAATGAATTGATACGAGATATCAAACGTCATCCTGAAGAAGGCATTGGTAAACCGGAAGCACTTCGTTTTGATTTGTCAG
GAAAATGGTCGCGCAGAATCAATCAGGAGCATAGACTGGTGTACCAAGTCATTGATGAGATGATAGTAATTTTTCAGTGT
CGTTATCATTATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References