Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2505637..2506275 Replicon chromosome
Accession NZ_CP046416
Organism Escherichia coli strain LZYWN_4

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GJV83_RS12290 Protein ID WP_000813794.1
Coordinates 2506099..2506275 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GJV83_RS12285 Protein ID WP_001270286.1
Coordinates 2505637..2506053 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJV83_RS12265 2500789..2501730 - 942 WP_001251304.1 ABC transporter permease -
GJV83_RS12270 2501731..2502744 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
GJV83_RS12275 2502762..2503907 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
GJV83_RS12280 2504152..2505558 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
GJV83_RS12285 2505637..2506053 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GJV83_RS12290 2506099..2506275 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GJV83_RS12295 2506497..2506727 + 231 WP_000494244.1 YncJ family protein -
GJV83_RS12300 2506819..2508780 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GJV83_RS12305 2508853..2509389 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
GJV83_RS12310 2509442..2510656 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T144244 WP_000813794.1 NZ_CP046416:c2506275-2506099 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T144244 NZ_CP061800:321922-323157 [Desulfonema magnum]
ATGAAAACACTTCGGGTGTTCCTGAACGACAGGCCGGTCGGCGAATTGCGCTCTGATAAAAAAAGAAGACTTGTTTTTCA
GTATCATCGGGAATATCTCGGAGCCTCGGACGCACATCCGCTCTCCCTGTCGCTTCCGCTCGGAGAATTACCTTTTGAGG
AAGATATCGCCCGTCCGTTTTTCTCCAATCTGCTTCCGGAGGGCGATATTCGGACGATCATTGCCAGGTTCGTCAGGGTG
TCAGGGAAGAACGACATGGCGCTTCTTGAAAAGATCGGCGGAGAGTGTGCCGGTGCTGTTTCCGTTCTGCCGGAGGGATT
CGTACCGGAGAAAAAAGGCGGCTACATTCGGCTTTCCCGGGATGAGCTTGATTCAATGATGGCGGCGGACACAGGCAGAC
CCCTGCTGATTAACCGGGAGGAGCTTCGGCTTTCCCTCGCGGGAGCCCAGGACAAACTGCCGGTCTTCATAAAAGACGGC
GAATATTTTCTTCCCACGGGGAACATGCCGAGTTCTCATATCATCAAACCCCGCAACCGCTATTTCGGGGGGACTGTGCA
GAATGAGGCGTTTTGCATGAAACTTGCCAAAGCCGCGGGGCTTCCGGTTCCCGGGAGTCATATCTGGAAAGGTGAGAAGA
ACATTGCCTATGTGGTGGAACGATATGACAGGGCGAGAGATGCGGAGGGAAATATCTCACGGATCCACCAGGAGGATTTC
TGCCAGGCCATGGGATATCTGCCGGATCAGAAATACGAAAGCGAGGGAGGCCCGGGTCTGGCGGAATGTTTTTCCCTGTT
GGAAAAGTTCAGCGCACAGCCGATCAGGGACAGGAAGAATTTTATCAGTTGGGTAATTTTTAACTTCCTTATCGGAAACG
CTGACGCACATGCGAAGAACATATCTCTCCTGTTCAGACACGGGAAAATATATCTTGCCCCGTTCTACGATCTCATGTCA
ACGCTTGTATATCCCGAACTGTCACAGAAGATGTCCATGAAGATCGGCAAGGAAAACCGCTACAAATGGACACGGAAAAG
GCATTGGGAGAGATTTGCAAGGGGGATCGGCATGAAACCCCGGTTTGTATTTTCGATTGCCGAGGATATGGCGGAAAGGC
TTAAAACAGAGTCGGCAGACATCGCAGGAGCGTTTGCTTCCTGGTACGCCGAAAAAGAACTGATTGAAAAGATAGGCAAT
ATTATTGAGAAGCATACTTCGTCTTTTGAATGGTGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT144244 WP_001270286.1 NZ_CP046416:c2506053-2505637 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT144244 NZ_CP061800:321683-321925 [Desulfonema magnum]
ATGAAAAAAATTTTCCCGAACGGTAATATTATGAATGACATAAAAGAAATCGGGTATATCATCAGAAAAAAACGCAGGGA
ATACGGACTCACACAGGTTGAAGCCGCTGGTCTGTGTAATGTGGGTGTCCGTTTCATGTCGGAACTTGAGAACGGAAAAC
CCACGCTTCAGGTGGGCAAGGTGCTGCATGTGCTGGAGGCATACGGATTTTCAGTGACAATTACCCACAGGGAAGATTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References