Detailed information of TA system TA144242

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2352191..2352717
• Replicon: chromosome
• Accession: NZ_CP046416
• Organism: Escherichia coli strain LZYWN_4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: GJV83_RS11545
• Protein ID: WP_000323025.1
• Coordinates: 2352430..2352717 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: GJV83_RS11540
• Protein ID: WP_000534858.1
• Coordinates: 2352191..2352430 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJV83_RS11505	2348260..2348370	+	111	WP_021531328.1	hypothetical protein	-
GJV83_RS11510	2348390..2349670	-	1281	WP_000876979.1	site-specific integrase	-
GJV83_RS11515	2349705..2349956	-	252	WP_000005552.1	excisionase family protein	-
GJV83_RS11520	2350029..2350373	-	345	WP_001487236.1	3'-5' exoribonuclease	-
GJV83_RS11525	2350441..2351138	+	698	Protein_2256	IS1-like element IS1A family transposase	-
GJV83_RS11530	2351326..2351658	-	333	WP_001301033.1	protein FlxA	-
GJV83_RS11535	2351861..2352166	-	306	WP_071593935.1	hypothetical protein	-
GJV83_RS11540	2352191..2352430	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
GJV83_RS11545	2352430..2352717	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
GJV83_RS11550	2352789..2352944	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
GJV83_RS11555	2353161..2353412	+	252	WP_001463433.1	hypothetical protein	-
GJV83_RS11560	2353479..2353757	+	279	WP_001515373.1	hypothetical protein	-
GJV83_RS11565	2353759..2354808	+	1050	WP_001515372.1	DUF968 domain-containing protein	-
GJV83_RS11570	2354826..2355203	+	378	WP_206970412.1	antitermination protein	-
GJV83_RS11575	2355359..2355883	-	525	WP_000780584.1	lipocalin family protein	-
GJV83_RS11580	2356076..2357035	+	960	WP_206970413.1	DUF1722 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2326426..2383596	57170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T144242 WP_000323025.1 NZ_CP046416:2352430-2352717 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T144242 NZ_CP061799:6343023-6343409 [Desulfonema limicola]
ATGAAACTTCTTTTAGATACACATGTTTTCCTTTGGTATATCAGCAAAGACAGCCGGCTGCCAAACGCAATGCTCGAAAA
CATACGAAATCCTGACAATGAAGTTTTTTTAAGCGTTGTATCTGTATGGGAGACAATTATCAAGTATCAGATTGGCAAAT
TACCGTTACCGCAAAGTCCTGAGATATATTTGCCAACCCAACGTAAACGTCATTCAATTTCAAGTTTGATTTTGGATGAA
TCAAGTGTCTGCCATCTTTCAAAACTGCCTTCTATTCATCGGGACCCTTTTGATCGAATACTTATTTGTCAGGCAATAGA
ACATGGATTAACAATTGCAACAATAGATAATGCAATCAAATCTTATGCTGTGAACATTTTGAAATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT144242 WP_000534858.1 NZ_CP046416:2352191-2352430 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT144242 NZ_CP061799:6342790-6343026 [Desulfonema limicola]
ATGATTTCTGTTACCATTGATGATATCCAGCAAGATTTACTTTATTATCTCAAACAAATAAATTTTGGTGAAACGGTTAT
AATTCTACAAGCTGATAAACCAATAGCAGAATTAAAACCGATTTCAGACTCAAAAATACTGCGTCCATTTGGTTTGTGTG
AGGGAGAATTCACTGTACCGGATGATTTTGATGAACCGCTGCCAGAGAATATAATTCAGGAATTTGAGGGACAATGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8