Detailed information of TA system TA144233

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1904479..1905310
• Replicon: chromosome
• Accession: NZ_CP046416
• Organism: Escherichia coli strain LZYWN_4

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: GJV83_RS09230
• Protein ID: WP_000854814.1
• Coordinates: 1904479..1904853 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: GJV83_RS09235
• Protein ID: WP_001285585.1
• Coordinates: 1904942..1905310 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJV83_RS09190	1900709..1901041	+	333	Protein_1800	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
GJV83_RS09195	1901160..1901633	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
GJV83_RS09200	1901831..1902889	+	1059	WP_001200891.1	FUSC family protein	-
GJV83_RS09205	1903061..1903390	+	330	WP_000450409.1	DUF496 family protein	-
GJV83_RS09210	1903491..1903673	-	183	WP_001016348.1	hypothetical protein	-
GJV83_RS09215	1903745..1903873	+	129	Protein_1805	transposase domain-containing protein	-
GJV83_RS09220	1904162..1904275	-	114	WP_001161660.1	DUF957 domain-containing protein	-
GJV83_RS09225	1904288..1904482	-	195	WP_000988600.1	hypothetical protein	-
GJV83_RS09230	1904479..1904853	-	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
GJV83_RS09235	1904942..1905310	-	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
GJV83_RS09240	1905384..1905605	-	222	WP_000692323.1	DUF987 domain-containing protein	-
GJV83_RS09245	1905668..1906144	-	477	WP_001186773.1	RadC family protein	-
GJV83_RS09250	1906160..1906639	-	480	WP_000860076.1	antirestriction protein	-
GJV83_RS09255	1906721..1907542	-	822	WP_001234530.1	DUF945 domain-containing protein	-
GJV83_RS09260	1907763..1908173	-	411	WP_000846713.1	hypothetical protein	-
GJV83_RS09265	1908189..1908872	-	684	WP_000775500.1	hypothetical protein	-
GJV83_RS09270	1909023..1909720	-	698	Protein_1816	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T144233 WP_000854814.1 NZ_CP046416:c1904853-1904479 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T144233 NZ_CP061799:423231-423512 [Desulfonema limicola]
ATGATTGTATCTTTTAAAAATAAAGAAACAGAGAAAATATTCAACCGCCGTTTTTCAAAAAAACTGCCTCATGATATACA
GCGGAAGGCTTTGGAAAAGTTATGGATGATAGATGTTGCAGATGATCTTTCCGCTTTACGGATTCCGCCTGGAAACCGGC
TGGAAGCATTGGAACATGATCGGAAAGGGCAGTACAGTATCCGGATAAACGATCAATGGCGCATATGCTTCAAATGGAAT
CGCAATAATGCTTACGATGTTGAAATTGAAGATTATCATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT144233 WP_001285585.1 NZ_CP046416:c1905310-1904942 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT144233 NZ_CP061799:423554-423853 [Desulfonema limicola]
ATGAATGAAGAAAAAATTCCGCCCATCCATCCTGGTGAAACTCTTTTGGGTGAATTTCTGGAACCCATGGGAATAAGTCG
TGCCAGGATTGCAAAATCTTTAAGTGTGCCGGAATCGGTTATCACTGATATTATACAGGGTAAAAAGTCTGTGACAGCAG
ATATTGCTCTGCGGCTTGGAAGGTATTTCAGAATGTCTCCCCAATTCTGGATGGGCCTGCAGAATCATTATGATCTGGCA
ATTGCCGAAGATGAACTGGAAAATCGGCTTGAGCAGGAAGTGGAACTTTATGCGGCATAA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60