Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 923262..923916 Replicon chromosome
Accession NZ_CP046416
Organism Escherichia coli strain LZYWN_4

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag GJV83_RS04460 Protein ID WP_000244777.1
Coordinates 923509..923916 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GJV83_RS04455 Protein ID WP_000354046.1
Coordinates 923262..923528 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJV83_RS04430 918431..919174 + 744 WP_000951964.1 SDR family oxidoreductase -
GJV83_RS04435 919231..920664 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
GJV83_RS04440 920709..921020 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
GJV83_RS04445 921184..921843 + 660 WP_000250274.1 hemolysin III family protein -
GJV83_RS04450 922039..923019 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
GJV83_RS04455 923262..923528 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GJV83_RS04460 923509..923916 + 408 WP_000244777.1 toxin CptA Toxin
GJV83_RS04465 923956..924477 - 522 WP_001055874.1 flavodoxin FldB -
GJV83_RS04470 924589..925485 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GJV83_RS04475 925510..926220 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GJV83_RS04480 926226..927959 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T144231 WP_000244777.1 NZ_CP046416:923509-923916 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T144231 NZ_CP061780:6360745-6361323 [Pseudomonas aeruginosa]
ATGTCCCTCGAACTGCTGAGCAACCGCCTCGAATTCCTCCGCGAAGCCGAACGCCTGAAAGACGTCCTGCGCTCCGCGCA
CACCTCCTCCGGCCGCCCGGAAAGCACCGCCGAACATAGCTGGCGGCTGTGCCTGATGGCACTGGCCTTCGAGGACCAAC
TGGCCGGCCTGGACCTCGGCAAGGTTCTGCGCATGTGCGTGGTCCACGACCTCGGCGAAGCGATCCACGGCGACATCCCC
GCCGTCGAGCAAGCGGCGCACCCGGACAAGGGCGAACAGGAACGCGCCGACCTGTTGCAGCTGACCCGTCATCTGGACAC
GCCGCTGCGCGATCGCCTGCTCGCCCTGTGGGACGAATACGAGCGCGGCGAGACCGCCGAAGCCCTGGCAGTGAAAGCGC
TGGACAAGCTGGAAACCCTGCTCCAGCACACCCAGGGCGACAATCCGGCGGACTTCGACTATGCCTTCAACCTCGACTAC
GGCCGTCGCTATACCGACCGCGCACCGCTGTTCAAGACCCTGCGCGAGCTACTCGACGCCAGGACCCGACAGCGCCTCGC
AGCTCGGCGCGATGCCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT144231 WP_000354046.1 NZ_CP046416:923262-923528 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT144231 NZ_CP061780:6360103-6360657 [Pseudomonas aeruginosa]
ATGACCGAGCCTCTCCCTTCTTCGTCCCTGGGCCCCGCCCTGCGTCGCTGGCGCCTGCTGCACCGGATCAAGCAAACGCA
TGCCGCCGAATTGCTCGGCGTGGCGCAATCGACCATCTCGCGCTGGGAGTCCGGTACCCAGGCGCTGCTGGTGGAGGAAC
GGGCACGCCTGGAGCGTCTGCTCGGCGCCCGCCTGGAAAGCGCCGCCGACCAGGCACTGGCCCGCCTGGTCGAGGCCAAC
CCGCAACCCGTGCACCTGATCTGCGACCTCACCCATCGCTTGCTGGCCTGTTCGCCGGCACGCGCCGCGCAATTCGGCGT
GCCTCTCGGCGAACTGCTCGACCGTTCGCTGTGGCCCTATTGCAGCGAGGAAATCCTCCGCCAGGAAGCCACCCTCGAGG
AACTCGGCTGGCGCGAACTGCTGGCACCGCCGGCGCTGGAGTTCGCCAGCGGAGCGAACGCCTCGGCCATCGTGCCGATC
CGGCGCAGCCGTTGCCGCTGGACCCGCATGACCCTCTCGGACGGACGCGCGGTGCGCCTGGTGGAAACCCTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References