Detailed information of TA system TA144213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3025806..3027027
• Replicon: chromosome
• Accession: NZ_CP046406
• Organism: Pseudomonas aeruginosa strain SE5458

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: V6AG16
• Locus tag: GNT62_RS14600
• Protein ID: WP_003088107.1
• Coordinates: 3025806..3026384 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GNT62_RS14605
• Protein ID: WP_003088112.1
• Coordinates: 3026473..3027027 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNT62_RS14585	3021082..3022359	+	1278	WP_003088104.1	TolC family protein	-
GNT62_RS14590	3022349..3024520	+	2172	WP_003088105.1	type I secretion system permease/ATPase	-
GNT62_RS14595	3024510..3025697	+	1188	WP_003088106.1	HlyD family efflux transporter periplasmic adaptor subunit	-
GNT62_RS14600	3025806..3026384	-	579	WP_003088107.1	HD domain-containing protein	Toxin
GNT62_RS14605	3026473..3027027	-	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
GNT62_RS14610	3027185..3029380	-	2196	WP_003088114.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
GNT62_RS14615	3029385..3029846	-	462	WP_003088117.1	(2Fe-2S)-binding protein	-
GNT62_RS14620	3030177..3030500	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
GNT62_RS14625	3030513..3030911	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
GNT62_RS14630	3030908..3031459	-	552	WP_023098678.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-187)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21885.74 Da
• Isoelectric Point: 5.3655

>T144213 WP_003088107.1 NZ_CP046406:c3026384-3025806 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDAKTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T144213 NZ_CP061778:c1879643-1879362 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTGGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGAACCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT144213 WP_003088112.1 NZ_CP046406:c3027027-3026473 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT144213 NZ_CP061778:c1879867-1879640 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: V6AG16