Detailed information of TA system TA144212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Acetyltransf_4(toxin)
• Location: 2032428..2033936
• Replicon: chromosome
• Accession: NZ_CP046406
• Organism: Pseudomonas aeruginosa strain SE5458

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GNT62_RS09795
• Protein ID: WP_073660237.1
• Coordinates: 2032428..2033015 (+)
• Length: 196 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A1C7C4U7
• Locus tag: GNT62_RS09800
• Protein ID: WP_003091607.1
• Coordinates: 2033082..2033936 (+)
• Length: 285 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNT62_RS09790	2028683..2032162	-	3480	WP_003091610.1	hybrid sensor histidine kinase/response regulator	-
GNT62_RS09795	2032428..2033015	+	588	WP_073660237.1	GNAT family N-acetyltransferase	Toxin
GNT62_RS09800	2033082..2033936	+	855	WP_003091607.1	AraC family transcriptional regulator	Antitoxin
GNT62_RS09805	2034033..2036198	+	2166	WP_003091606.1	TonB-dependent receptor family protein	-
GNT62_RS09810	2036373..2038247	+	1875	WP_003091605.1	MFS transporter	-
GNT62_RS09815	2038308..2038517	-	210	WP_003091604.1	cold-shock protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (20-152)

Antitoxin

• AraC-type arabinose-binding/dimerisation domain (19-151)

Sequences

Toxin

Protein

• Length: 196 a.a.
• Molecular weight: 21929.29 Da
• Isoelectric Point: 9.8676

>T144212 WP_073660237.1 NZ_CP046406:2032428-2033015 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLVEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Nucleotide

• Length: 588 bp

>T144212 NZ_CP061778:1797048-1797341 [Pseudomonas aeruginosa]
ATGCCACAAGTGATCCTCTCTCCGGCTGCTCTGCGTGACCTTAAACGGCTGCGCGAGTTCTTGCACAGTAAGAGCCCAGT
TGTTGCACAGCGAGCAGCCCAAACCATCCGTTCGGCGCTGGCCCAGCTCGGGCAACAACCCGCGATGGGGCGTCCTATTG
AAGGGCTCCCGGAGGTGTTCCGCGAGTGGGTCATCCCCTTTGGGGACAGTGGTTATCTGGCGCGTTACCGCCTAGAGGGC
GAAACCGTAGTGATCTTGGCTCTGCGTCACCAGCGCGAAGCCGGGTTTATTTAG

Antitoxin

Protein

• Length: 285 a.a.
• Molecular weight: 31895.20 Da
• Isoelectric Point: 7.5295

>AT144212 WP_003091607.1 NZ_CP046406:2033082-2033936 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETMLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTTYE

Nucleotide

• Length: 855 bp

>AT144212 NZ_CP061778:1796773-1797060 [Pseudomonas aeruginosa]
ATGTCCGCAGCTACCAAACCCATGTCTATCAAACTTGATAGCGATACCCAAAGCCGGATCAAGCAGCTAGCTGCTGCCCG
CGACCGTTCAGCGCACTGGATGATGCGCGAAGCTATCACCCAGTATGTTGAGCGCGAAGAGCGCAAGGAGGCGTTCCGCC
GCGATACCCTCGATGCTTGGCAGGAGTTTCAGGAGACTGGTTTGCATGTCTCTCTTGAGGAGACCGATCAGTGGTTGGCT
GGTTGGGGCACCGAGATTGAAGGTGGTGCACCTGCATGCCACAAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1C7C4U7