Detailed information of TA system TA144209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 4503271..4503857
• Replicon: chromosome
• Accession: NZ_CP046405
• Organism: Pseudomonas aeruginosa strain SE5443

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: GNT61_RS20770
• Protein ID: WP_003120987.1
• Coordinates: 4503558..4503857 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: GNT61_RS20765
• Protein ID: WP_003448662.1
• Coordinates: 4503271..4503561 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNT61_RS20750	4498853..4500742	+	1890	WP_179063445.1	hypothetical protein	-
GNT61_RS20755	4500739..4502715	+	1977	WP_016851611.1	DEAD/DEAH box helicase	-
GNT61_RS20760	4502856..4503200	+	345	WP_016851612.1	hypothetical protein	-
GNT61_RS20765	4503271..4503561	-	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
GNT61_RS20770	4503558..4503857	-	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GNT61_RS20775	4504059..4505183	+	1125	WP_012076859.1	TcpQ domain-containing protein	-
GNT61_RS20780	4505183..4506892	+	1710	WP_012076860.1	PilN family type IVB pilus formation outer membrane protein	-
GNT61_RS20785	4506896..4508221	+	1326	WP_003099758.1	type 4b pilus protein PilO2	-
GNT61_RS20790	4508211..4508744	+	534	WP_023094340.1	type IV pilus biogenesis protein PilP	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4469740..4556182	86442

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T144209 WP_003120987.1 NZ_CP046405:c4503857-4503558 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T144209 NZ_CP061778:c209674-209384 [Pseudomonas aeruginosa]
ATGACCGAGCAAATTTATGATTATGACCCCGCGCAAGCGCTGGACAGCCCGGAGGCCATGGCTCTGTTCATCGCAGACGC
CTTGGATACTGGAGACACCGCCTATATCGCTAAGGCTATGGGAGTCGTGGCTCGCGCCAAGGGGATGACGGAGTTGGCCA
AGGAAACGGGTTTGGCCAGGGAGCAGCTTTACAAATCGTTTAGTGAGCGCGGGAACCCGACATTGAAAACCATGCTGGCA
GTAATGCGAGCTTTGGGCGTTGATCTGACCGCCCGCCCGCATGCTCAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT144209 WP_003448662.1 NZ_CP046405:c4503561-4503271 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT144209 NZ_CP061778:c209970-209671 [Pseudomonas aeruginosa]
ATGGTTGAAGTCAAACAAACCGCCACTTTCATGGCATGGGAAAGCAAGCTAAAGGATCGGCGGGCAAAGGCCGTGATCGC
CGCCCGTATCTTCCGATTGGCGAATGGACTACCCGGCGATGTGTCACCAGTCGGCCAGGGTGTGAGCGAGTTGAGGATTC
ACTATGGCCCAGGCTATCGGGTGTACTTCCAGCAGCGCGGGACGGAAATCGTCATCCTGCTATGTGGTGGAGACAAGAGC
AGCCAGGCCAGGGATATAGAAATGGCGAAACGCCTGGCAAATGAGTGGAGGCCGCAATGA

Structures

Toxin

• AlphaFold DB: G8CP73