Detailed information of TA system TA144208

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4412751..4414008
• Replicon: chromosome
• Accession: NZ_CP046405
• Organism: Pseudomonas aeruginosa strain SE5443

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: GNT61_RS20315
• Protein ID: WP_003449003.1
• Coordinates: 4412751..4413506 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: GNT61_RS20320
• Protein ID: WP_003086329.1
• Coordinates: 4413640..4414008 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNT61_RS20305	4407924..4410467	+	2544	WP_003140632.1	acyl-homoserine-lactone acylase QuiP	-
GNT61_RS20310	4410622..4411983	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
GNT61_RS20315	4412751..4413506	-	756	WP_003449003.1	RES family NAD+ phosphorylase	Toxin
GNT61_RS20320	4413640..4414008	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
GNT61_RS20325	4414081..4415367	-	1287	WP_003112529.1	FAD-binding oxidoreductase	-
GNT61_RS20330	4415423..4417012	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
GNT61_RS20335	4417091..4417567	+	477	WP_003140638.1	hypothetical protein	-
GNT61_RS20340	4417570..4417845	-	276	WP_010792073.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28292.45 Da
• Isoelectric Point: 7.7294

>T144208 WP_003449003.1 NZ_CP046405:c4413506-4412751 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGTAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T144208 NZ_CP061777:c6991582-6991301 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACGCCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCCCTGAAAGCCGTCCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCTTTTCGGGTAAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT144208 WP_003086329.1 NZ_CP046405:c4414008-4413640 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT144208 NZ_CP061777:c6991806-6991579 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCGTATCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACACCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Antitoxin

• AlphaFold DB: V6AJU7