Detailed information of TA system TA144207

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3493938..3495159
• Replicon: chromosome
• Accession: NZ_CP046405
• Organism: Pseudomonas aeruginosa strain SE5443

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: GNT61_RS16020
• Protein ID: WP_003102740.1
• Coordinates: 3494581..3495159 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GNT61_RS16015
• Protein ID: WP_003088112.1
• Coordinates: 3493938..3494492 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNT61_RS15990	3489506..3490057	+	552	WP_003450159.1	XRE family transcriptional regulator	-
GNT61_RS15995	3490054..3490452	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
GNT61_RS16000	3490465..3490788	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
GNT61_RS16005	3491119..3491580	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
GNT61_RS16010	3491585..3493780	+	2196	WP_014603034.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
GNT61_RS16015	3493938..3494492	+	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
GNT61_RS16020	3494581..3495159	+	579	WP_003102740.1	HD domain-containing protein	Toxin
GNT61_RS16025	3495268..3496455	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
GNT61_RS16030	3496445..3498616	-	2172	WP_003114950.1	type I secretion system permease/ATPase	-
GNT61_RS16035	3498606..3499883	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T144207 WP_003102740.1 NZ_CP046405:3494581-3495159 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T144207 NZ_CP061777:c5700251-5699973 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT144207 WP_003088112.1 NZ_CP046405:3493938-3494492 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT144207 NZ_CP061777:c5699961-5699656 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A0C7CS07