Detailed information of TA system TA144195

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5882674..5883314
• Replicon: chromosome
• Accession: NZ_CP046403
• Organism: Pseudomonas aeruginosa strain SE5369

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GNT46_RS27810
• Protein ID: WP_003134109.1
• Coordinates: 5882674..5883084 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GNT46_RS27815
• Protein ID: WP_031634724.1
• Coordinates: 5883084..5883314 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNT46_RS27770	5878800..5878997	+	198	WP_019396765.1	hypothetical protein	-
GNT46_RS27775	5879154..5879882	+	729	WP_004352842.1	TIGR03761 family integrating conjugative element protein	-
GNT46_RS27780	5879888..5880436	+	549	WP_004352841.1	DUF3158 family protein	-
GNT46_RS27785	5880483..5881322	+	840	WP_003105750.1	Rha family transcriptional regulator	-
GNT46_RS27790	5881352..5881840	+	489	WP_004352840.1	single-stranded DNA-binding protein	-
GNT46_RS27795	5881996..5882163	+	168	WP_128729268.1	CrpP family protein	-
GNT46_RS27800	5882229..5882447	-	219	WP_023083218.1	hypothetical protein	-
GNT46_RS27805	5882461..5882658	-	198	WP_023083219.1	hypothetical protein	-
GNT46_RS27810	5882674..5883084	-	411	WP_003134109.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GNT46_RS27815	5883084..5883314	-	231	WP_031634724.1	antitoxin	Antitoxin
GNT46_RS27820	5883570..5885489	+	1920	WP_004352838.1	type I DNA topoisomerase	-
GNT46_RS27825	5885797..5886006	+	210	WP_003105733.1	cold-shock protein	-
GNT46_RS27830	5886227..5888116	+	1890	WP_003105732.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5864317..5955787	91470

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-33)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15452.78 Da
• Isoelectric Point: 7.3233

>T144195 WP_003134109.1 NZ_CP046403:c5883084-5882674 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVTPIV

Nucleotide

• Length: 411 bp

>T144195 NZ_CP061764:3849674-3850015 [Escherichia coli O19:H7]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8420.29 Da
• Isoelectric Point: 4.2821

>AT144195 WP_031634724.1 NZ_CP046403:c5883314-5883084 [Pseudomonas aeruginosa]
VEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVAVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT144195 NZ_CP061764:3849336-3849653 [Escherichia coli O19:H7]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA