Detailed information of TA system TA144194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 5057380..5058638
• Replicon: chromosome
• Accession: NZ_CP046403
• Organism: Pseudomonas aeruginosa strain SE5369

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9I4U4
• Locus tag: GNT46_RS23900
• Protein ID: WP_003112528.1
• Coordinates: 5057380..5058135 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: GNT46_RS23905
• Protein ID: WP_003086329.1
• Coordinates: 5058270..5058638 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNT46_RS23890	5052553..5055096	+	2544	WP_003112526.1	acyl-homoserine-lactone acylase QuiP	-
GNT46_RS23895	5055251..5056612	-	1362	WP_073647918.1	DNA recombination protein RmuC	-
GNT46_RS23900	5057380..5058135	-	756	WP_003112528.1	RES family NAD+ phosphorylase	Toxin
GNT46_RS23905	5058270..5058638	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
GNT46_RS23910	5058711..5059997	-	1287	WP_023083139.1	FAD-binding oxidoreductase	-
GNT46_RS23915	5060053..5061642	-	1590	WP_003125055.1	aldehyde dehydrogenase family protein	-
GNT46_RS23920	5061721..5062197	+	477	WP_003112531.1	hypothetical protein	-
GNT46_RS23925	5062200..5062475	-	276	WP_169057979.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28278.42 Da
• Isoelectric Point: 7.7294

>T144194 WP_003112528.1 NZ_CP046403:c5058135-5057380 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMVVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T144194 NZ_CP061764:3684702-3684920 [Escherichia coli O19:H7]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT144194 WP_003086329.1 NZ_CP046403:c5058638-5058270 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT144194 NZ_CP061764:3684302-3684676 [Escherichia coli O19:H7]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: Q9I4U4

Antitoxin

• AlphaFold DB: V6AJU7