Detailed information of TA system TA144193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 4065444..4066665
• Replicon: chromosome
• Accession: NZ_CP046403
• Organism: Pseudomonas aeruginosa strain SE5369

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GNT46_RS19075
• Protein ID: WP_003114949.1
• Coordinates: 4066087..4066665 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GNT46_RS19070
• Protein ID: WP_003088112.1
• Coordinates: 4065444..4065998 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNT46_RS19045	4061012..4061563	+	552	WP_003102727.1	XRE family transcriptional regulator	-
GNT46_RS19050	4061560..4061958	-	399	WP_023082958.1	NADH-quinone oxidoreductase subunit A	-
GNT46_RS19055	4061971..4062294	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
GNT46_RS19060	4062625..4063086	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
GNT46_RS19065	4063091..4065286	+	2196	WP_023082959.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
GNT46_RS19070	4065444..4065998	+	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
GNT46_RS19075	4066087..4066665	+	579	WP_003114949.1	HD domain-containing protein	Toxin
GNT46_RS19080	4066773..4067960	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
GNT46_RS19085	4067950..4070121	-	2172	WP_003088105.1	type I secretion system permease/ATPase	-
GNT46_RS19090	4070111..4071388	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21897.75 Da
• Isoelectric Point: 5.3655

>T144193 WP_003114949.1 NZ_CP046403:4066087-4066665 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAVRRDA

Nucleotide

• Length: 579 bp

>T144193 NZ_CP061764:2897646-2897753 [Escherichia coli O19:H7]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTACTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT144193 WP_003088112.1 NZ_CP046403:4065444-4065998 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT144193 NZ_CP061764:c2897596-2897533 [Escherichia coli O19:H7]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT