Detailed information of TA system TA144192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3020319..3021361
• Replicon: chromosome
• Accession: NZ_CP046403
• Organism: Pseudomonas aeruginosa strain SE5369

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: GNT46_RS14505
• Protein ID: WP_003153636.1
• Coordinates: 3020786..3021361 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: GNT46_RS14500
• Protein ID: WP_003050245.1
• Coordinates: 3020319..3020789 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNT46_RS14465	3015711..3017129	-	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-
GNT46_RS14470	3017119..3018030	-	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
GNT46_RS14475	3018027..3018719	-	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
GNT46_RS14480	3018716..3019114	-	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
GNT46_RS14485	3019126..3019485	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
GNT46_RS14490	3019502..3019735	-	234	WP_006226027.1	TIGR03758 family integrating conjugative element protein	-
GNT46_RS14495	3019732..3020115	-	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
GNT46_RS14500	3020319..3020789	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
GNT46_RS14505	3020786..3021361	+	576	WP_003153636.1	PIN domain-containing protein	Toxin
GNT46_RS14510	3021379..3022293	+	915	WP_016852809.1	AAA family ATPase	-
GNT46_RS14515	3022290..3022760	+	471	WP_003090160.1	hypothetical protein	-
GNT46_RS14520	3022757..3023257	+	501	WP_003090159.1	hypothetical protein	-
GNT46_RS14525	3023257..3024159	+	903	WP_003090158.1	nucleotidyltransferase domain-containing protein	-
GNT46_RS14530	3024198..3024923	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2943117..3067477	124360

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T144192 WP_003153636.1 NZ_CP046403:3020786-3021361 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T144192 NZ_CP061764:2897111-2897218 [Escherichia coli O19:H7]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT144192 WP_003050245.1 NZ_CP046403:3020319-3020789 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT144192 NZ_CP061764:c2897064-2896998 [Escherichia coli O19:H7]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• AlphaFold DB: I3TV68