Detailed information of TA system TA144187

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4926958..4928216
• Replicon: chromosome
• Accession: NZ_CP046402
• Organism: Pseudomonas aeruginosa strain SE5331

Toxin (Protein)

• Gene name: -
• Uniprot ID: V6AJ34
• Locus tag: GNT44_RS23265
• Protein ID: WP_003086331.1
• Coordinates: 4926958..4927713 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: GNT44_RS23270
• Protein ID: WP_003086329.1
• Coordinates: 4927848..4928216 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNT44_RS23255	4922129..4924672	+	2544	WP_003086335.1	acyl-homoserine-lactone acylase QuiP	-
GNT44_RS23260	4924827..4926188	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
GNT44_RS23265	4926958..4927713	-	756	WP_003086331.1	RES family NAD+ phosphorylase	Toxin
GNT44_RS23270	4927848..4928216	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
GNT44_RS23275	4928289..4929575	-	1287	WP_003086327.1	FAD-binding oxidoreductase	-
GNT44_RS23280	4929631..4931220	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
GNT44_RS23285	4931299..4931775	+	477	WP_003086322.1	hypothetical protein	-
GNT44_RS23290	4931778..4932053	-	276	WP_023098713.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28236.34 Da
• Isoelectric Point: 7.7294

>T144187 WP_003086331.1 NZ_CP046402:c4927713-4926958 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T144187 NZ_CP061764:1932037-1932288 [Escherichia coli O19:H7]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT144187 WP_003086329.1 NZ_CP046402:c4928216-4927848 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT144187 NZ_CP061764:1932285-1932539 [Escherichia coli O19:H7]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: V6AJ34

Antitoxin

• AlphaFold DB: V6AJU7