Detailed information of TA system TA144186

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3917100..3918321
• Replicon: chromosome
• Accession: NZ_CP046402
• Organism: Pseudomonas aeruginosa strain SE5331

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: V6AG16
• Locus tag: GNT44_RS18330
• Protein ID: WP_003088107.1
• Coordinates: 3917743..3918321 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GNT44_RS18325
• Protein ID: WP_003088112.1
• Coordinates: 3917100..3917654 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNT44_RS18300	3912668..3913219	+	552	WP_023098678.1	XRE family transcriptional regulator	-
GNT44_RS18305	3913216..3913614	-	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
GNT44_RS18310	3913627..3913950	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
GNT44_RS18315	3914281..3914742	+	462	WP_003088117.1	(2Fe-2S)-binding protein	-
GNT44_RS18320	3914747..3916942	+	2196	WP_003088114.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
GNT44_RS18325	3917100..3917654	+	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
GNT44_RS18330	3917743..3918321	+	579	WP_003088107.1	HD domain-containing protein	Toxin
GNT44_RS18335	3918430..3919617	-	1188	WP_003088106.1	HlyD family efflux transporter periplasmic adaptor subunit	-
GNT44_RS18340	3919607..3921778	-	2172	WP_003088105.1	type I secretion system permease/ATPase	-
GNT44_RS18345	3921768..3923045	-	1278	WP_003088104.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-187)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21885.74 Da
• Isoelectric Point: 5.3655

>T144186 WP_003088107.1 NZ_CP046402:3917743-3918321 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDAKTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T144186 NZ_CP061764:1180303-1180614 [Escherichia coli O19:H7]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT144186 WP_003088112.1 NZ_CP046402:3917100-3917654 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT144186 NZ_CP061764:1180611-1181030 [Escherichia coli O19:H7]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: V6AG16