Detailed information of TA system TA144181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2285812..2286790
• Replicon: chromosome
• Accession: NZ_CP046398
• Organism: Bacillus sp. A260

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: GD442_RS11670
• Protein ID: WP_000624977.1
• Coordinates: 2285812..2286549 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: GD442_RS11675
• Protein ID: WP_000237819.1
• Coordinates: 2286662..2286790 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GD442_RS11645	2281425..2281832	+	408	WP_000072495.1	VOC family protein	-
GD442_RS11650	2281856..2282236	+	381	WP_000497668.1	methyltransferase	-
GD442_RS11655	2282394..2284079	-	1686	WP_002041140.1	alpha-keto acid decarboxylase family protein	-
GD442_RS11660	2284187..2284669	+	483	WP_000191885.1	MarR family winged helix-turn-helix transcriptional regulator	-
GD442_RS11665	2284836..2285573	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
GD442_RS11670	2285812..2286549	+	738	WP_000624977.1	stage II sporulation protein SA	Toxin
GD442_RS11675	2286662..2286790	+	129	WP_000237819.1	hypothetical protein	Antitoxin
GD442_RS11680	2286863..2287039	+	177	WP_000852629.1	stage II sporulation protein SB	-
GD442_RS11685	2287055..2287444	-	390	WP_000713869.1	YxeA family protein	-
GD442_RS11690	2287656..2289125	+	1470	WP_000287546.1	beta-Ala-His dipeptidase	-
GD442_RS11695	2289371..2290180	+	810	WP_000664544.1	C39 family peptidase	-
GD442_RS11700	2290206..2290808	+	603	WP_000517259.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T144181 WP_000624977.1 NZ_CP046398:2285812-2286549 [Bacillus sp. A260]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T144181 NZ_CP061764:c628023-627559 [Escherichia coli O19:H7]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT144181 WP_000237819.1 NZ_CP046398:2286662-2286790 [Bacillus sp. A260]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT144181 NZ_CP061764:c628358-628023 [Escherichia coli O19:H7]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2