Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 236039..236681 Replicon chromosome
Accession NZ_CP046398
Organism Bacillus sp. A260

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag GD442_RS01340 Protein ID WP_000635963.1
Coordinates 236331..236681 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag GD442_RS01335 Protein ID WP_000004570.1
Coordinates 236039..236326 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GD442_RS01310 231356..232318 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
GD442_RS01315 232311..232883 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
GD442_RS01320 232977..233336 + 360 WP_000583416.1 holo-ACP synthase -
GD442_RS01325 233493..234443 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
GD442_RS01330 234561..235730 + 1170 WP_000390615.1 alanine racemase -
GD442_RS01335 236039..236326 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
GD442_RS01340 236331..236681 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GD442_RS01345 236749..238917 + 2169 WP_000426242.1 RNA-binding transcriptional accessory protein -
GD442_RS01350 238975..239091 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
GD442_RS01355 239287..239745 + 459 WP_071740807.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T144180 WP_000635963.1 NZ_CP046398:236331-236681 [Bacillus sp. A260]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T144180 NZ_CP046398:236331-236681 [Bacillus sp. A260]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT144180 WP_000004570.1 NZ_CP046398:236039-236326 [Bacillus sp. A260]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT144180 NZ_CP046398:236039-236326 [Bacillus sp. A260]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References