Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4495798..4496295 Replicon chromosome
Accession NZ_CP046396
Organism Escherichia coli strain ampC_0069

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GNX12_RS21475 Protein ID WP_001173343.1
Coordinates 4495798..4495971 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GNX12_RS21480 Protein ID WP_000398619.1
Coordinates 4495999..4496295 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GNX12_RS21465 4492468..4493925 + 1458 WP_022646457.1 dipeptide/tripeptide permease DtpC -
GNX12_RS21470 4494162..4495679 + 1518 WP_022646456.1 lysine--tRNA ligase -
GNX12_RS21475 4495798..4495971 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GNX12_RS21480 4495999..4496295 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GNX12_RS21485 4496524..4496796 - 273 WP_000405647.1 N-acetyltransferase -
GNX12_RS21490 4496808..4497038 - 231 WP_001683478.1 (4Fe-4S)-binding protein -
GNX12_RS21495 4497219..4498850 + 1632 WP_022646455.1 sensor histidine kinase -
GNX12_RS21500 4498847..4499566 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4486944..4496295 9351


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T144177 WP_001173343.1 NZ_CP046396:c4495971-4495798 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T144177 NZ_CP061758:c4313163-4312990 [Escherichia coli O68:H12]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT144177 WP_000398619.1 NZ_CP046396:c4496295-4495999 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT144177 NZ_CP061758:c4313487-4313191 [Escherichia coli O68:H12]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References