Detailed information of TA system TA144176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4394061..4394656
• Replicon: chromosome
• Accession: NZ_CP046396
• Organism: Escherichia coli strain ampC_0069

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: GNX12_RS20965
• Protein ID: WP_000239581.1
• Coordinates: 4394061..4394411 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: GNX12_RS20970
• Protein ID: WP_001223213.1
• Coordinates: 4394405..4394656 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GNX12_RS20945	4389515..4390537	-	1023	WP_001298067.1	ABC transporter permease	-
GNX12_RS20950	4390551..4392053	-	1503	WP_022646474.1	sugar ABC transporter ATP-binding protein	-
GNX12_RS20955	4392186..4393142	-	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
GNX12_RS20960	4393452..4393982	+	531	WP_000055075.1	inorganic diphosphatase	-
GNX12_RS20965	4394061..4394411	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
GNX12_RS20970	4394405..4394656	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
GNX12_RS20975	4394868..4395209	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
GNX12_RS20980	4395212..4398991	-	3780	WP_022646473.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T144176 WP_000239581.1 NZ_CP046396:c4394411-4394061 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T144176 NZ_CP061758:3767373-3767651 [Escherichia coli O68:H12]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT144176 WP_001223213.1 NZ_CP046396:c4394656-4394405 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT144176 NZ_CP061758:3767110-3767370 [Escherichia coli O68:H12]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7