Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4281721..4282297 Replicon chromosome
Accession NZ_CP046396
Organism Escherichia coli strain ampC_0069

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag GNX12_RS20410 Protein ID WP_001297643.1
Coordinates 4282010..4282297 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag GNX12_RS20405 Protein ID WP_000063156.1
Coordinates 4281721..4282023 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GNX12_RS20390 4278317..4280467 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
GNX12_RS20395 4280517..4280720 + 204 WP_000467859.1 YbdD/YjiX family protein -
GNX12_RS20400 4280731..4281687 + 957 WP_001297640.1 GTPase -
GNX12_RS20405 4281721..4282023 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
GNX12_RS20410 4282010..4282297 - 288 WP_001297643.1 BrnT family toxin Toxin
GNX12_RS20415 4282496..4283193 - 698 Protein_3998 IS1 family transposase -
GNX12_RS20420 4283250..4283345 + 96 Protein_3999 dienelactone hydrolase family protein -
GNX12_RS20425 4283491..4284723 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
GNX12_RS20430 4284764..4286044 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4282496..4282705 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T144175 WP_001297643.1 NZ_CP046396:c4282297-4282010 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T144175 NZ_CP061758:c3761311-3760913 [Escherichia coli O68:H12]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT144175 WP_000063156.1 NZ_CP046396:c4282023-4281721 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT144175 NZ_CP061758:c3761607-3761314 [Escherichia coli O68:H12]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References