Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4174547..4175097 Replicon chromosome
Accession NZ_CP046396
Organism Escherichia coli strain ampC_0069

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag GNX12_RS19890 Protein ID WP_001160966.1
Coordinates 4174547..4174861 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag GNX12_RS19895 Protein ID WP_000125568.1
Coordinates 4174864..4175097 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GNX12_RS19865 4170222..4171508 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
GNX12_RS19870 4171508..4172497 + 990 WP_000241224.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
GNX12_RS19875 4172494..4173315 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
GNX12_RS19880 4173318..4173695 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
GNX12_RS19885 4173702..4174550 + 849 WP_000257195.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
GNX12_RS19890 4174547..4174861 - 315 WP_001160966.1 CcdB family protein Toxin
GNX12_RS19895 4174864..4175097 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
GNX12_RS19900 4175183..4175662 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
GNX12_RS19905 4175854..4177716 - 1863 WP_022645165.1 glutathione-regulated potassium-efflux system protein KefC -
GNX12_RS19910 4177709..4178239 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
GNX12_RS19915 4178347..4179678 - 1332 WP_001183201.1 MFS transporter -
GNX12_RS19920 4179736..4180023 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T144174 WP_001160966.1 NZ_CP046396:c4174861-4174547 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T144174 NZ_CP061758:3555513-3555731 [Escherichia coli O68:H12]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT144174 WP_000125568.1 NZ_CP046396:c4175097-4174864 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT144174 NZ_CP061758:3555113-3555487 [Escherichia coli O68:H12]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References