Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 3765681..3766319 Replicon chromosome
Accession NZ_CP046396
Organism Escherichia coli strain ampC_0069

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag GNX12_RS17965 Protein ID WP_000813794.1
Coordinates 3765681..3765857 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag GNX12_RS17970 Protein ID WP_001270286.1
Coordinates 3765903..3766319 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GNX12_RS17945 3761303..3762514 - 1212 WP_071788120.1 BenE family transporter YdcO -
GNX12_RS17950 3762567..3763103 + 537 WP_000429148.1 DNA-binding transcriptional regulator SutR -
GNX12_RS17955 3763176..3765137 + 1962 WP_023909195.1 23S rRNA 5-hydroxycytidine C2501 synthase -
GNX12_RS17960 3765229..3765459 - 231 WP_000491567.1 DUF2554 family protein -
GNX12_RS17965 3765681..3765857 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
GNX12_RS17970 3765903..3766319 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
GNX12_RS17975 3766398..3767804 + 1407 WP_022645665.1 PLP-dependent aminotransferase family protein -
GNX12_RS17980 3768049..3769194 + 1146 WP_000047466.1 ABC transporter substrate-binding protein -
GNX12_RS17985 3769212..3770225 + 1014 WP_022645666.1 ABC transporter ATP-binding protein -
GNX12_RS17990 3770226..3771167 + 942 WP_022645667.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T144172 WP_000813794.1 NZ_CP046396:3765681-3765857 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T144172 NZ_CP061758:2689520-2689627 [Escherichia coli O68:H12]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT144172 WP_001270286.1 NZ_CP046396:3765903-3766319 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT144172 NZ_CP061758:c2689473-2689407 [Escherichia coli O68:H12]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References