Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3362858..3363642 Replicon chromosome
Accession NZ_CP046396
Organism Escherichia coli strain ampC_0069

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag GNX12_RS15985 Protein ID WP_000613626.1
Coordinates 3362858..3363352 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID A0A0A1AEM7
Locus tag GNX12_RS15990 Protein ID WP_024946541.1
Coordinates 3363349..3363642 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GNX12_RS15965 3358051..3359148 + 1098 WP_022645522.1 flagellar basal body P-ring protein FlgI -
GNX12_RS15970 3359148..3360089 + 942 WP_001317765.1 flagellar assembly peptidoglycan hydrolase FlgJ -
GNX12_RS15975 3360155..3361798 + 1644 WP_022645523.1 flagellar hook-associated protein FlgK -
GNX12_RS15980 3361810..3362763 + 954 WP_022645524.1 flagellar hook-associated protein FlgL -
GNX12_RS15985 3362858..3363352 - 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
GNX12_RS15990 3363349..3363642 - 294 WP_024946541.1 DUF1778 domain-containing protein Antitoxin
GNX12_RS15995 3363776..3366949 - 3174 WP_022645526.1 ribonuclease E -
GNX12_RS16000 3367522..3368481 + 960 WP_022645527.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T144169 WP_000613626.1 NZ_CP046396:c3363352-3362858 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T144169 NZ_CP061758:2078239-2078342 [Escherichia coli O68:H12]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11039.60 Da        Isoelectric Point: 10.5423

>AT144169 WP_024946541.1 NZ_CP046396:c3363642-3363349 [Escherichia coli]
MAAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRSEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT144169 NZ_CP061758:c2078380-2078109 [Escherichia coli O68:H12]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB A0A0A1AEM7

References